این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Public Health، جلد ۳۷، شماره ۴، صفحات ۵۹-۶۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Detection of Leishmania major In Naturally Infected Sand Flies Using Semi Nested-PCR
چکیده انگلیسی مقاله Background: The aim of this study was to assess Leishmania infection in sand fly species from areas where leishmaniasis is endemic. This is important for prediction of the risk and expansion of the disease. Methods: In this cross-sectional study we used a PCR-based method for detection of Leishmania minicircle DNA within individual sand flies from Orzoieh, a new endemic leishmaniasis focus in southern Iran. Results: We detected minicircle DNA in 6 of 92 (6.5%) Phlebotomus (Phlebotomus) papatasi collected indoor, while all of previous microscopic examination of sand flies specimens was negative for Leishmania promastigotes in the region. The species were identified as Leishmania (Leishmania) major by comparison of PCR products with a L. major positive control. All the Leishmania -positive sand flies were confirmed as P. (P.) papatasi by using a morphological key of Iranian sand flies. Conclusion: Since PCR method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of Leishmania species as well as monitoring the infection rate in sand fly populations in areas of low endemicity of leishmaniasis.
کلیدواژه‌های انگلیسی مقاله Leishmania major, Sand fly, Leishmaniasis, PCR, Iran
نویسندگان مقاله ma عشاقی | ma oshaghi
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te


mr یعقوبی ارشادی | mr yaghobi ershadi
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te


m عباسی | m abbassi
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te


p پرویزی | p parvizi
dept. of parasitology, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

aa اخوان | aa akhavan
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te


a رحیمی فروشانی | a rahimi foroshani
dept. of biostatics amp;amp; epidemiology, school of public health, tehran university of medical sciences,

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ar زهرایی | ar zahraei
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te


راثی | y rassi
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te


f محترمی | f mohtarami
dept. of medical entomology and vector control, faculty of health amp;amp; institute of health research, te
نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/2015
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات