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Iranian Journal of Public Health، جلد ۳۷، شماره ۴، صفحات ۱۱۴-۱۱۸
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عنوان انگلیسی Detection of Mutation in 23S rRNA in Isolates of Streptococcus pyogenes Resistant to Erythromycin
چکیده انگلیسی مقاله Background: In this study, erythromycin resistance isolates from the students with aged 12-15 yr old were studied and the mutations in the 23S rRNA were identified by sequencing. Methods: Throat samples of 327 students with 12-15 years old from 3 schools of Tehran were cultured on the selective Strep­tococ­cus pyogenes medium and 23 strains were primarily selected as S. pyogenes . Results: All strains were susceptible to penicillin and bacitracin (0.04U). Minimum inhibitory of concentrations (MICs) for eryth­romycin were determined in which 5 strains were found intermediate, 2 strains resistance and the remaining 16 strains susceptible to erythromycin. MIC for the erythromycin-resistance strains, BT 3 and BT 4 , were 8.2 and 12.4 µg/ml, respec­tively. PCR was performed for each six 23S rRNA operons and a fragment with 550 bp was sequenced. For two resis­tance strains, BT 3 and BT 4 there were a mutation in position 2059 (A to G). Also another mutation in position 2529 (G to A) was detected in BT3. Conclusion: In addition to mutation at the position 2058 other point mutations in different positions of domain V involve in the resistance to macrolides. 
کلیدواژه‌های انگلیسی مقاله Streptococcus pyogenes, 23S rRNA, erythromycin-resistance
نویسندگان مقاله z sepehrizadeh | z sepehrizadeh
dept. of pharmaceutical biotechnology, faculty of pharmacy, tehran university of medical sciences,

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

g زرینی | g zarrini
dept. of animal sciences, faculty of natural sciences university of tabriz, iran

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

m بحرالعلومی شاپورآبادی | m bahrololoumi shapourabadi
dept. of pharmaceutical biotechnology, faculty of pharmacy, tehran university of medical sciences,

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m طباطبایی یزدی | m tabatabaei yazdi
dept. of pharmaceutical biotechnology, faculty of pharmacy, tehran university of medical sciences,

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m حسینی شاد | m hosseini shad
dept. of pharmaceutical biotechnology, faculty of pharmacy, tehran university of medical sciences,

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/2023
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