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Iranian Journal of Public Health، جلد ۳۷، شماره ۳، صفحات ۱۳۴-۱۴۱
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عنوان انگلیسی Detection of Aflr Gene and Toxigenicity of Aspergillus flavus Group Isolated from Patients with Fungal Sinusitis
چکیده انگلیسی مقاله Background: Aspergillus flavus is the second most important Aspergillus species causing human infections particu­larly fun­gal sinusitis . Since little is known about aflatoxin producing ability of clinical isolates, this study was under­taken to de­tect the aflatoxigenic isolates amongst these isolates. Methods: A total of 23 isolates of A. spp. which were recovered from patients proved to have fungal sinusitis by morpho­logi­cal and histological methods and also 5 additional aflatoxigenic and non-aflatoxigenic reference of A. fla­vus group strains were studied. The isolates were identified morphologically using Czapek Yeast Agar and A. flavus and parasiticus Agar (AFPA). Aflatoxin producing ability of the isolates was confirmed by Thin Layer Chromatogra­phy. Existing of aflR gene the regulatory gene in aflatoxin biosynthesis, were studied in all isolates by PCR method. Results: All twenty three Aspergillus isolates confirmed as A. flavus group by their macroscopic and microscopic fea­tures. One clinical isolate confirmed as A. oryzae by mycological methods. A. oryzae as well as A. flavus JCM2061 and NCPF2008 and 3 clinical isolates were not able to produce orange pigment on AFPA. From total of 23 iso­lates 4 (17.4%) con­firmed to be aflatoxigenic by TLC method. A banding pattern which matched to aflR primers was amplified with approxi­mate size of 800 bp in all 23 clinical A. flavus isolates. A larger banding pattern 1050 bp was revealed in clinical iso­late; strain no.20 as well. Conclusion: Some clinical sinus isolates are able to produce aflatoxin and all of studied isolates including; A. oryzae, A. parasiti­cus and A. sojae were able to amplify aflR gene under our laboratory conditions.
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نویسندگان مقاله p دهقان | p dehghan
dept. of parasitology amp;amp; mycology, school of public health and institute of public health research, t


زینی | f zaini
dept. of parasitology amp;amp; mycology, school of public health and institute of public health research, t


رضایی | s rezaei
dept. of parasitology amp;amp; mycology, school of public health and institute of public health research, t


a جبالی | a jebali
dept. of parasitology amp;amp; mycology, school of public health and institute of public health research, t


پریوش کردبچه | p kordbacheh
dept. of parasitology amp;amp; mycology, school of public health and institute of public health research, t


محمودی | m mahmoudi
dept. of epidemiology and biostatics, school of public health and institute of public health researc
نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/2046
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