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Journal of Medical Bacteriology، جلد ۵، شماره ۱-۲، صفحات ۲۹-۳۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Study of Antimicrobial Activities of Partially Purified Cyclotide Content and Crude Extracts from Viola tricolor
چکیده انگلیسی مقاله Background : Searching for the potent natural antibiotics will improve the treatment of most infectious diseases. Cyclotides are potent active peptides derived from some plant families like Violaceae. Viola tricolor (the pansy) has been found to have numerous cyclotides. Nowadays cyclotides attract more attention for their antibacterial activities. The current research studied the antimicrobial properties of semi-purified cyclotides from V. tricolor . Methods : Extraction and purification of cyclotides from Iranian Viola tricolor were performed by fractionation and solid phase extraction methods, and their antimicrobial effects were studied against several bacterial strains using diffusion assays. Also, attendance of cyclotide in the extract was verified by Tricine-SDS page and spectroscopic methods. Results : Antimicrobial effects of semi-purified cyclotides and crude extracts resulted in the antibacterial activity potential of V. tricolor totally extracted samples against gram negative bacteria, E.coli and P. aeruginosa . However, there is need for optimizing the assay method and the culture media. Conclusion : Viola tricolor as a remedy represents the antibacterial potential, which may not be unrelated to its cyclotide content although the effectiveness of cyclotides may also differ because of their synergism, natural structure and bioactivities, the amount of purified content, and the way they were assayed. 
کلیدواژه‌های انگلیسی مقاله Viola tricolor,Cyclotide,Extraction,Antibacterial effect
نویسندگان مقاله زهرا خوشکام | zahra khoshkam
department of biotechnology, faculty of pharmacy, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محبوبه ضرابی | mahboubeh zarabi
department of biotechnology, faculty of pharmacy, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ضرغام سپهری زاده | zargham sepehri zade
department of plant sciences, faculty of biological sciences, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مریم کشاورزی | maryam keshavarzi

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/228
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/313/article-313-442065.pdf
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زبان مقاله منتشر شده en
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