این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Physiology and Pharmacology، جلد ۲۱، شماره ۳، صفحات ۲۵۱-۲۵۹
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عنوان انگلیسی Single administrations of high and low doses of acetaminophen causes different effects on COX-2 gene expression and on tissue damage in liver and kidneys
چکیده انگلیسی مقاله Introduction: High dose of acetaminophen (APAP) is known to have hepatotoxic and nephrotoxic effects and studies show that this toxicities are dependent on the function of phase I bioactivation enzymes- Cyp450- and phase II biotransformation enzymes especially glucuronosylation and sulfonation pathways. However, the role of cyclooxygenase (COX) as an inflammatory mediator in toxic effects of APAP has not been explained satisfactorily yet. Methods: In this study, we aimed to find out if there is any association between APAP hepatotoxicity and COX-2 expression at mRNA levels. Male Balb/C mice were treated with a single high dose (300 mg/kg BW) or low dose (30 mg/kg BW) of APAP. Results: Following APAP treatment, serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzymes were measured as the biochemical markers of hepatocellular damage. Then liver and kidney biopsies were processed and examined for histolopathogical changes as well as for total RNA extraction and COX-2 gene expression. Serum ALT/AST levels were significantly (P< 0.05) higher and there were hepatotoxic damages after 24 hours in mice exposed to high dose of APAP (300 mg/kg BW). However, no obvious nephrotoxicity was observed in mice treated with either low or high doses of APAP. Based on RT-PCR data, the COX-2 specific mRNA was not expressed in liver tissues of either control or APAP-treated mice, while, it was expressed in kidney tissues of both control and APAP-treated mice. Conclusion: These data may suggest that unlike in liver, COX-2 expression in kidney may play a protective role in APAP-related hepatotoxicity.
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نویسندگان مقاله مرتضی حاتم زاده خانقاهی | morteza hatamzadeh khaneghahi
department of biology, kurdistan science and research branch, islamic azad university, sanandaj, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

سرور شجاعیان | sorour shojaeian
department of clinical biochemistry, medical genetics, nutrition, alborz university of medical sciences, karaj, iran


عبدالامیر علامه | abdolamir allameh
department of clinical biochemistry, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://phypha.ir/ppj/browse.php?a_code=A-10-1000-1&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/26/article-26-445666.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Others
نوع مقاله منتشر شده Original Research
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