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فصلنامه علمی پژوهشی گیاهان دارویی، جلد ۳، شماره ۶۳، صفحات ۹۲-۱۰۷
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Phytochemical Evaluation and Plant Regeneration Through Somatic Embryogenesis of Ferulago angulata subsp. Carduchorum (Boiss. and Hausskn), an Endangered Medicinal Plant
چکیده انگلیسی مقاله Background: Ferulago angulata is an important medicinal plant and endemic to Iran which is an endangered Plant. Objective: The aim of this study was to achieve an efficient method for propagation of this plant and evaluation of essential oil content and composition in habitat. Metthods: Essential oils were extracted by hydrodistillation using Clevenger-type apparatus and its composition was analyzed by the GC and GC-MS. For callus induction, explants were cultured on MS medium supplemented with NAA, 2,4-D and BA. For somatic embryogenesis, the calli were cultured on MS medium supplemented with full, 1/4 and 1/10 fold of growth regulators and MS medium without growth regulator. For maturation, embryogenic clumps were transferred on MS medium supplemented with BA, 2,4-D and 30 or 60 g l-1 sucrose. The small embryogenic clumps were transferred to medium with GA3. Results: The main components of essential oils were Ocimene (29.84%), followed by α-pinene (27.01%), Isobornyl formate (7.7%) and gamma-Terpinene (4.85%). The highest percentage of callus induction observed in the root and hypocotyl explants. The maximum somatic embryogenesis was obtained in MS medium with 0.5 mg l-1 2, 4-D via hypocotyls derived explants. The highest frequency of maturation was obtained in MS medium containing 30 g l-1 sucrose without growth regulators. Conclution: This plant had a noticeable amount of essential oil with valuable ingredients and tissue culture techniques could be applied for its mass propagation, conservation of germplasm and in vitro regeneration.
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نویسندگان مقاله h نقدی بادی | h naghdi badi
1- medicinal plants research center, institute of medicinal plants, acecr, karaj, iran

سازمان اصلی تایید شده: پژوهشکده‮ ‬گیاهان‮ ‬دارویی‮ ‬جهاد‮ ‬دانشگاهی (Institute of medicinal plants)

s سالک هاشمی | s salek hashemi
2- department of plant breeding and biotechnology, gorgan university of agricultural science and natural resources, gorgan, iran

سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

a نصراله نژاد قمی | a nasrollahnezhad qomi
2- department of plant breeding and biotechnology, gorgan university of agricultural science and natural resources, gorgan, iran

سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

s نواب پور | s navabpour
2- department of plant breeding and biotechnology, gorgan university of agricultural science and natural resources, gorgan, iran

سازمان اصلی تایید شده: دانشگاه گلستان (Golestan university)

a قادری | a qaderi
1- medicinal plants research center, institute of medicinal plants, acecr, karaj, iran

سازمان اصلی تایید شده: پژوهشکده‮ ‬گیاهان‮ ‬دارویی‮ ‬جهاد‮ ‬دانشگاهی (Institute of medicinal plants)

نشانی اینترنتی http://jmp.ir/browse.php?a_code=A-10-75-9&slc_lang=en&sid=fa
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/88/article-88-448250.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده بیوتکنولوژی
نوع مقاله منتشر شده پژوهشی
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