این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
فصلنامه علمی پژوهشی گیاهان دارویی، جلد ۳، شماره ۶۳، صفحات ۱۱۹-۱۳۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Diversity Evaluation of Trigonella foenum-graecum Populations Using DNA Markers and Phytochemical Characteristics
چکیده انگلیسی مقاله Background: Fenugreek (Trigonella foenum-graecum L.) is an important medicinal plant that is a source of diosgenin, trigonelline and other valuable components. Fenugreek seeds is used for its pharmaceutical and nutraceutical properties. Objective: The aim of present study was to investigate the genetic diversity within and between different populations of Trigonella foenum-graecum using DNA markers and phytochemical characteristics. Methods: The total genomic DNA was isolated from 12 populations of Trigonella foenum-graecum collected from different regions in Iran. After optimizing PCR, 10 ISSR and 10 SCoT primers were used to amplify the genomic DNA of all genotypes. To investigate genetic distance, cluster analysis based on Jaccard similarity coefficients and UPGMA algorithm calculated for the 12 genotypes. Also, the trigonelline content was determined between 2 populations which had the highest genetic distance. Results: Analysis of banding patterns of 10 SCoT primers revealed 92 polymorphic bands with an average of 9.2 fragments per prime. A total of 79 bands were generated from 10 ISSR primers, out of which 55 (68.21%) were polymorphic bands. The average values of dissimilarities based on the SCoT and ISSR data were 0.60 and 0.44, respectively. Also, shoot trigonelline content was significantly different between genotypes with the highest genetic distance.  Conclusion: Our results revealed a high level of genetic variation among fenugreek populations. The findings also indicated that SCoT technique is more informative than ISSR technique for evaluation of genetic diversity and relationships among fenugreek populations. The genotypes with the highest genetic distance based on SCoT data, were noticeably different in respect of trigonelline content.   
کلیدواژه‌های انگلیسی مقاله Trigonella foenum-graecum L., Genetic variation, ISSR, SCoT
نویسندگان مقاله h دانشمند | h daneshmand
1- department of biotechnology and plant breeding, kermanshah branch, islamic azad university, kermanshah, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

ar اطمینان | ar etminan
1- department of biotechnology and plant breeding, kermanshah branch, islamic azad university, kermanshah, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

a قادری | a qaderi
2- medicinal plants research center, institute of medicinal plants, acecr, karaj, iran

سازمان اصلی تایید شده: پژوهشکده‮ ‬گیاهان‮ ‬دارویی‮ ‬جهاد‮ ‬دانشگاهی (Institute of medicinal plants)

نشانی اینترنتی http://jmp.ir/browse.php?a_code=A-10-472-5&slc_lang=en&sid=fa
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/88/article-88-448252.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده بیوتکنولوژی
نوع مقاله منتشر شده پژوهشی
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات