این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Pediatric Hematology and Oncology، جلد ۵، شماره ۳، صفحات ۱۷۲-۱۸۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Gene Expression and Promoter Methylation Status of VHL, Runx-3, E-cadherin, P15 and P16 Genes During EPO-Mediated Erythroid Differentiation of CD34+ Hematopoietic Stem Cells
چکیده انگلیسی مقاله Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage. Materials and Methods: CD34+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system. Expanded CD34+ stem cells were differentiated into erythroid lineage with human recombinant erythropoietin (EPO). DNA extraction was done by QIAamp DNA Mini Kit. RNA was extracted using RNase Mini plus Kit. MSP (Methylation specific PCR) technique was done for methylation assay. Methylation status and expression assay was done for VHL, Runx-3, E-cadherin, P15, and P16 genes on both CD34+ stem cells and differentiated erythroid cells. Results: The results showed that, before differentiation, P15 had comparative methylation pattern and average expression and it remained unchanged after differentiation (p=0.01). concerning P16, results revealed no methylation pattern and complete expression in absence of EPO and with EPO it changed to comparative status (p=0.01). E-cad and Runx-3 genes had relative methylation pattern and fully expression before and after differentiation but their expression after that, was increased and decreased Respectively (p=0.04). VHL gene had no significant methylation status before or after differentiation and its expression was complete (p=0.01). Conclusion: The obtained results indicated that promoter methylation of P15, P16, VHL, Runx3 and E-cad was one of the definitive expression control mechanism of these genes.
کلیدواژه‌های انگلیسی مقاله Differentiation, EPO, Hematopoietic Stem Cells
نویسندگان مقاله فاطمه اسکندری | fatemeh eskandari
department of hematology, school of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

هدی پورکریم | hoda pourkarim
department of hematology, allied medical school, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

امیرحسین پاکپور | amir h pakpour
. social determinants of health research center, qazvin university of medical sciences, qazvin, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی گودرزی | mehdi goudarzi
. department of microbiology, school of medicine, shahid beheshti university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

ناصر مبرا | naser mobarra
5. stem cell research center, school of medicine, golestan university of medical sciences, gorgan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی سهمانی | mehdi sahmani
department of clinical biochemistry, cellular and molecular research center, qazvin, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

علی دهقانی فرد | ali dehghanifard
department of hematology, school of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

نسیم کلانتری | nasim kalantari
department of hematology, school of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

خمیسی پور غلامرضا | khamisipour gholamreza
department of hematology, faculty of allied medicine, bushehr university of medical sciences, bushehr, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

مهدی آزاد | mehdi azad
department of medical laboratory sciences, faculty of allied medicine, qazvin university of medical sciences, qazvin, ir

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بوشهر (Bushehr university of medical sciences)

نشانی اینترنتی http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-70-90&slc_lang=fa&sid=en
فایل مقاله دریافت فایل مقاله
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات