این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Archives of Hygiene Sciences، جلد ۶، شماره ۳، صفحات ۲۳۵-۲۴۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Evaluation of Heavy Metals Resistance in Biofilm Cells of Native Rhodococcus spp. Isolated from Soil
چکیده انگلیسی مقاله Background & Aims of the Study: Rhodococci according to possess large genome, their active metabolism and survival under extreme conditions, are highly regarded for biodegradation and bioremediation of different pollutants especially heavy metals in the environment. Biofilms are more resistant to heavy metals than free-swimming organisms. The aim of this study was biofilm formation of two native Rhodococcus strains (Rhodococcus rhodochrous and Rhodococcus rhodnii) and comparative evaluation effects of heavy metals such as lead, copper, zinc, chromium and cadmium in various concentrations against biofilm and planktonic cells that carried out at the first time. Materials & Methods: Bacterial strains used in this investigation were isolated from agricultural soils in Qom, Iran. Biofilm formation was performed at 30 °C and 37 °C in tryptic soy broth (TSB) and brain heart infusion (BHI) broth, at 24,48,72 and 96 hours in 96-well microplate. Minimum inhibitory concentration (MIC) of heavy metals in various concentrations for planktonic cells was determined, using microdilution and macrodilution methods. Minimum biofilm inhibitory concentration (MBIC) and Minimum biofilm eradicating concentration (MBEC) in various concentrations of heavy metals for biofilm cells were evaluated by microdilution method. The result of this study confirmed, using scanning electron microscope (SEM). Results: The best condition for biofilm formation of native R.rhodochrous isolate was determined after incubation at 37°C in BHI broth, at 96 hours and the best condition for biofilm formation of native R.rhodnii isolate was determined at 30°C in BHI broth, at 96 hours. MIC of heavy metals for planktonic cells of isolates for cadmium, zinc and lead was 8 mM and assayed for copper and chromium respectively 4 and 1 mM. MBEC of heavy metals for biofilm cells of isolates for cadmium, zinc and lead was 16 mM and assayed for copper and chromium respectively 8 and 4 mM. The results of present study showed biofilm cells of native Rhodococcus isolates were 2 times more resistance to lead, copper, zinc and cadmium than planktonic cells while biofilm cells were 4 times more resistance than planktonic cells to chromium. Conclusions: Biofilm formation of R.rhodochrous and R.rhodnii and their high resistance to various concentrations of heavy metals especially cadmium show that inoculation of these native Rhodococcus isolates to contaminated agricultural soils with heavy metals, have an effective role for bioremediation.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله الهام ادهمی | elham adhami
department of microbiology, qom branch, islamic azad university, qom, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

سهیل آقایی | soheil aghaei
department of microbiology, qom branch, islamic azad university, qom, iran.
دانشگاه آزاد اسلامی، قم، ایران.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی قم (Islamic azad university of ghom)

محمد رضا ذوالفقاری | mohammad reza zolfaghari
islamic azad university, qom, iran.
دانشگاه آزاد اسلامی، قم، ایران.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی قم (Islamic azad university of ghom)

نشانی اینترنتی http://jhygiene.muq.ac.ir/browse.php?a_code=A-10-95-1&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/251/article-251-449431.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات