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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۹، صفحات ۹۹۳-۱۰۰۲
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عنوان انگلیسی Anti-inflammatory effect of Yu-Ping-Feng-San via TGF-β1 signaling suppression in rat model of COPD
چکیده انگلیسی مقاله Objective(s): Yu-Ping-Feng-San (YPFS) is a classical traditional Chinese medicine that is widely used for treatment of the diseases in respiratory systems, including chronic obstructive pulmonary disease (COPD) recognized as chronic inflammatory disease. However, the molecular mechanism remains unclear. Here we detected the factors involved in transforming growth factor beta 1 (TGF-β1)/Smad2 signaling pathway and inflammatory cytokines, to clarify whether YPFS could attenuate inflammatory response dependent on TGF-β1/Smad2 signaling in COPD rats or cigarette smoke extract (CSE)-treated human bronchial epithelial (Beas-2B) cells. Materials and Methods: The COPD rat model was established by exposure to cigarette smoke and intratracheal instillation of lipopolysaccharide, YPFS was administered to the animals. The efficacy of YPFS was evaluated by comparing the severity of pulmonary pathological damage, pro-inflammation cytokines, collagen related genes and the activation of TGF-β1/Smad2 signaling pathway. Furthermore, CSE-treated cells were employed to confirm whether the effect of YPFS was dependent on the TGF-β1/Smad2 signaling via knockdown Smad2 (Si-RNA), or pretreatment with the inhibitor of TGF-β1. Results: Administration of YPFS effectively alleviated injury of lung, suppressed releasing of pro-inflammatory cytokines and collagen deposition in COPD animals (P< 0.05), whereas exogenous TGF-β1 promoted releasing of IL-1β, IL-6, TNFα (P< 0.05). Administration YPFS reduced inflammatory response significantly, also down-regulated TGF-β1/Smad2 signaling in vivo and in vitro. Unexpectedly, knockdown Smad2 or inhibition of TGF-β1 abolished anti-inflammatory effect of YPFS in CSE-treated cells. Conclusion: YPFS accomplished anti-inflammatory effects mainly by suppressing phosphorylation of Smad2, TGF-β1/Smad2 signaling pathway was required for YPFS-mediated anti-inflammation in COPD rats or CSE-treated Beas-2B cells.
کلیدواژه‌های انگلیسی مقاله COPD, Pro-inflammatory, Cytokine, TGF-β1/Smad2, YPFS
نویسندگان مقاله zhong shan یانگ | zhong shan yang
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan


جین yuan یان | jin yuan yan
central laboratory, the second affiliated hospital of kunming medical university, kunming, yunnan


ni ping هانیه | ni ping han
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan


wei zhou | wei zhou
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan


yu چنگ | yu cheng
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan


محمد بن همام رییس کنفدراسیون mei زانگ | xiao mei zhang
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan


ning li | ning li
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan


جیا li yuan | jia li yuan
faculty of basic medical science, yunnan university of traditional chinese medicine, kunming, yunnan
نشانی اینترنتی http://ijbms.mums.ac.ir/article_7600.html
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