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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۷، صفحات ۷۵۵-۷۶۲
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عنوان انگلیسی Methylation and mRNA expression levels of P15, death-associated protein kinase, and suppressor of cytokine signaling-1 genes in multiple myeloma
چکیده انگلیسی مقاله Objective(s): The aim of this study was to investigate the methylation status and mRNA expression levels of P15, death-associated protein kinase (DAPK), and suppressor of cytokine signaling-1 (SOCS1) genes in multiple myeloma (MM). Materials and Methods: The bone marrow samples of 54 MM patients were collected and the methylation status of the P15, DAPK, and SOCS1 gene promoter regions was determined by methylation-specific polymerase chain reaction. Automated sequencing technology was used to sequence the amplified products in order to analyze the base methylation sites. mRNA expression levels were determined using real-time fluorescent quantitative polymerase chain reaction . Results: Among the 54 MM patients, the positive methylation rates of the P15, DAPK, and SOCS1 genes were 27.78%, 18.52%, and 16.67%, respectively. The methylation results were confirmed by sequencing. The positive methylation rates of the P15, DAPK, and SOCS1 genes showed no correlation with patient gender, age, typing, staging, and grouping (P>0.05). There was no significant difference in the mRNA expression levels of the P15, DAPK, and SOCS1 genes between the MM patient group and the control group (P>0.05). Conclusions: Aberrant methylation of the P15, DAPK, and SOCS1 genes exists in MM, and these genes may play certain roles in pathogenesis of MM. There was no significant difference in mRNA expression levels between the methylated group and the non-methylated group, suggesting that these genes are regulated by other mechanisms during their transcription.
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نویسندگان مقاله lin liu | lin liu
department of hematology, the second affiliated hospital of kunming medical university, kunming 650101, china


lin tan | lin tan
department of hematology, the first affiliated hospital of kunming medical university, kunming 650041, china


zhenxin he | zhenxin he
department of hematology, the first affiliated hospital of kunming medical university, kunming 650041, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_7361.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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