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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۷، صفحات ۷۹۴-۷۹۹
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عنوان انگلیسی Immune cell distribution and immunoglobulin levels change following sciatic nerve injury in a rat model
چکیده انگلیسی مقاله Objective(s): To investigate the systemic and local immune status of two surgical rat models of sciatic nerve injury, a crushed sciatic nerve, and a sciatic nerve transection Materials and Methods:Twenty-four adult male Sprague-Dawley rats were randomly divided into three groups: sham-operation (control group), sciatic nerve crush, and sciatic nerve transaction. Sciatic nerve surgery was performed. The percentage of CD4+ cells and the CD4+/CD8+ratio were determined by flow cytometry. Serum IgM and IgG levels were analyzed by ELISA. T-cells (CD3) and macrophages (CD68) in sciatic nerve tissue sections were identified through immunohistochemistry. Results: Compared to sham-operated controls, in rats that underwent nerve injury, the percentage of CD4+ cells and the CD4+/CD8+ ratio in the peripheral blood were significantly decreased 7 days after surgery, serum IgM levels were increased 14 days after surgery, and serum IgG levels were increased 21 days after surgery. There were a large number of CD3+ cells and a small number of CD68+ cells in sciatic nerve tissue sections 21 days after surgery, indicating T-cell and macrophage activation and infiltration. Local IgG deposition was also detected at the nerve injury site 21 days after surgery. Conclusion: Rat humoral and cellular immune status changed following sciatic nerve injury, particularly with regard to the cellular immune response at the nerve injury site.
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نویسندگان مقاله wei yuan | wei yuan
department of spine surgery, aviation general hospital of china medical university, beijing institute of translational medicine, chinese academy of sciences, no. 3 anwai beiyuan road, chaoyang district, beijing, 100012, china


xinhong feng | xinhong feng
department of neurology, beijing tsinghua changgung hospital medical center, tsinghua university, no. 168 li tang rd. dongxiaokou town, tiantongyuan area, changping district, beijing, 102218, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_7366.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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