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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۶، صفحات ۶۳۸-۶۴۵
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عنوان انگلیسی Study of chondrogenic potential of stem cells in co-culture with chondrons
چکیده انگلیسی مقاله Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production. Materials and Methods: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (900 nm fiber diameter) was obtained from Bon Yakhteh (Tehran- Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP- ASCs on scaffolds were co-cultured with articular chondrons using transwell. After 21 day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen2, aggrecan and Indian hedgehog using real- time RT-PCR. Results: Genes expression of collagen2, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P˂ 0.05). Conclusion: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture.
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نویسندگان مقاله پریسا نیک پو | parisa nikpou
department of anatomical sciences, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

داریوش محمدنژاد | daryoush mohammad nejad
drug applied research center, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

هاجر شفایی | hajar shafaei
department of anatomical sciences, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

لیلا روشنگر | leila roshangar
department of anatomical sciences, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

ناصر صمدی | nasser samadi
department of biochemistry and laboratory sciences, faculty of advanced medical sciences, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

امیر محمد نوالی | amir mohammad navali
department of orthopedy, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

علیرضا صادقپور | ali reza sadegpour
department of orthopedy, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

داریوش شانه بندی | dariush shanehbandi
department of immunolog, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

جعفر سلیمانی راد | jafar soleimani rad
department of anatomical sciences, faculty of medicine, tabriz university of medical sciences, tabriz, iran|umblical cord stem cell research center, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_7132.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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