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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۵، صفحات ۴۸۳-۴۸۹
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عنوان انگلیسی The protective effects of epigallocatechin gallate on lipopolysa ccharide-induced hepatotoxicity: an in vitro study on Hep3B cells
چکیده انگلیسی مقاله Objective(s): In the present study, our aim was to investigate the possible protective effects of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS)-induced hepatotoxicity by using Hep3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line Hep3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells. Materials and Methods:The Hep3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses. Results:The highest treatment dose of EGCG (400 µM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 µM) normalized all related parameters levels. Conclusion:LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways.
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نویسندگان مقاله مرات karamese | murat karamese
department of medical microbiology, medical faculty, kafkas university, kars, turkey


bulent guvendi | bulent guvendi
department of general surgery, medical faculty, kafkas university, kars, turkey


selina aksak karamese | selina aksak karamese
department of histology and embryology, medical faculty, kafkas university, kars, turkey


عرفان cinar | irfan cinar
department of pharmacology, medical faculty, ataturk university, erzurum, turkey


serpil can | serpil can
department of physiology, medical faculty, kafkas university, kars, turkey


huseyin serkan erol | huseyin serkan erol
department of biochemistry, veterinary faculty, ataturk university, erzurum, turkey


حاکان آیدین | hakan aydin
department of virology, veterinary faculty, ataturk university, erzurum, turkey


volkan gelen | volkan gelen
department of physiology, veterinary faculty, kafkas university, kars, turkey


emre کاراکوس | emre karakus
department of pharmacology and toxicology, veterinary faculty, ataturk university, erzurum, turkey
نشانی اینترنتی http://ijbms.mums.ac.ir/article_6932.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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