این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۳، صفحات ۲۸۱-۲۸۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Diagnosis of genetic defects through parallel assessment of PLCζ and CAPZA3 in infertile men with history of failed oocyte activation
چکیده انگلیسی مقاله Objective(s): Phospholipase C ζ (PLCζ) is considered as a nominee for sperm associated oocyte activating factors and is located back-to-back with CAPZA3, an actin-capping protein controlling actin polymerization during spermiogenesis. They contain a common bidirectional promoter. The objective of this study was to identify individuals with parallel low expression of PLCζ and CAPZA3 mRNA, in hope of detecting genetic defects in this bidirectional promoter. Materials and Methods: Semen samples were collected from 24 fertile and 59 infertile individuals with total failed, low and high fertilization rate post intra-cytoplasmic sperm injection (ICSI), as well as globozoospermic individuals.Expression of PLCζ and CAPZA3 were assessed by Real time PCR. In addition, PLCζ was assessed by Western blot. Results: Significant correlations between PLCζ with CAPZA3 and also between these two genes with fertilization were observed. Individuals with low fertilization presented significantly lower expression of these two genes. Low expression of PLCζ was also verified by Western analysis. Sequence analysis of bidirectional promoter of these two genes in an individual with parallel low expression of both PLCζ and CAPZA3, revealed a mutation within the CAPZA3 predicted promoter, known as human regulatory factor X4 which is a testis-specific dimeric DNA-binding protein. In the opposite stand, in the same location, the mutation appears to be outside but in the vicinity of PLCζ, in a binding region predicate by Genomatix. Conclusion: Parallel assessment of CAPZA3 with PLCζ at mRNA level in individuals with inability to induce oocyte activation may help researcher to identify genetic defects associated with failed fertilization.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله سودابه جوادیان الیادرانی | soudabeh javadian elyaderani
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, acecr, isfahan, iran|department of biology, science and research branch, islamic azad university, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

کامران قایدی | kamran ghaedi
department of molecular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran|biology department, school of sciences, university of isfahan, isfahan, iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

مرضیه تولایی | marziyeh tavalaee
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, acecr, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

فرزانه ربیعی | farzaneh rabiee
department of molecular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

محمدرضا دیمه | mohammad reza deemeh
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, acecr, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani
department of reproductive biotechnology, reproductive biomedicine research center, royan institute for biotechnology, acecr, isfahan, iran|department of molecular biotechnology, cell science research center, royan institute for biotechnology, acecr, isfahan, iran|isfahan fertility and infertility center, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_6647.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات