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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۲، صفحات ۱۲۵-۱۳۱
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عنوان انگلیسی Berberine suppresses migration of MCF-7 breast cancer cells through down-regulation of chemokine receptors
چکیده انگلیسی مقاله Objective(s): Berberine is one of the main alkaloids and it has been proven to have different pharmacological effects including inhibition of cell cycle and progression of apoptosis in various cancerous cells; however, its effects on cancer metastasis are not well known. Cancer cells obtain the ability to change their chemokine system and convert into metastatic cells. In this study, we examined the effect of berberine on breast cancer cell migration and its probable interaction with the chemokine system in cancer cells. Materials and Methods: The MCF-7 breast cancer cell line was cultured, and then, treated with berberine (10, 20, 40 and 80 μg/ml) for 24 hr. MTT assay was used in order to determine the cytotoxic effect of berberine on MCF-7 breast cancer cells. Wound healing assay was applied to determine the inhibitory effect of berberine on cell migration. Moreover, real-time quantitative PCR analysis of selected chemokine receptors was performed to determine the probable molecular mechanism underlying the effect of berberine on breast cancer cell migration. Results: The results of wound healing assay revealed that berberine decreases cell migration. Moreover, we found that the mRNA levels of some chemokine receptors were reduced after berberine treatment, and this may be the underlying mechanism for decreased cell migration. Conclusion: Our results indicate that berberine might be a potential preventive biofactor for human breast cancer metastasis by targeting chemokine receptor genes.
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نویسندگان مقاله نغمه ahmadiankia | naghmeh ahmadiankia
shahroud university of medical sciences, shahroud, iran


حمید کلالیان مقدم | hamid kalalian moghaddam
shahroud university of medical sciences, shahroud, iran


محمد امیر mishan | mohammad amir mishan
department of biology, ferdowsi university of mashhad, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

احمدرضا بهرامی | ahmad reza bahrami
department of biology, ferdowsi university of mashhad, mashhad, iran|cell and molecular biotechnology research group, institute of biotechnology, ferdowsi university of mashhad, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

حجت نادری مشکین | hojjat naderi meshkin
stem cell and regenerative medicine research group, iranian academic center for education, culture and research acecr , mashhad branch, mashhad, iran


حمیدرضا بیدخوری | hamid reza bidkhori
department of biology, ferdowsi university of mashhad, mashhad, iran|stem cell and regenerative medicine research group, iranian academic center for education, culture and research acecr , mashhad branch, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

مریم مقدم | maryam moghaddam
department of biology, school of basic sciences, islamic azad university of damghan, damghan, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی دامغان (Islamic azad university of damghan)

سید جمال الدین میرفیضی | seyed jamal aldin mirfeyzi
department of biology, school of basic sciences, islamic azad university of damghan, damghan, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی دامغان (Islamic azad university of damghan)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_6531.html
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