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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۱۲، صفحات ۱۲۴۰-۱۲۴۴
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چکیده فارسی مقاله
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عنوان انگلیسی Screening and identification of SUMP-proteins in sub-acute treatment with diazinon
چکیده انگلیسی مقاله Objective(s):Small ubiquitin-like modifiers (SUMOs) are a family of ubiquitin-related, proteins that are involved in a wide variety of signaling pathways. SUMOylation, as a vital post translational modification, regulate protein function in manycellular processes. Diazinon (DZN), an organophosphate insecticide, causses oxidative stress and subsequently programmed cell death in different tissues. The aim of this study was to evaluate the role and pattern of SUMO modificationas a defense mechanism against stress oxidative, in the heart tissuesof the DZN treated rats. Materials and Methods: Diazinon (15 mg/kg/day), corn oil (control) were administered via gavageto male Wistar rats for four weeks. SUMO1 antibody was covalently crosslinked to protein A/G agarose. heart tissue lysate were added to agarosebeads,After isolation of target proteins(SUMO1- protein)SDS-PAGE gel electrophoresis was performed. Protein bands were identified using MALDI-TOF/TOF and MASCOT). Fold change of (DZN/Ctrl) separated proteins was evaluated using UVband software (UVITEC, UK). Results:Our result showed that subacute exposure to DZN increased SUMOylationoffour key proteins involved in the metabolic process including; Acyl-CoA dehydrogenase, creatine kinase, glyceraldehyde-3-phosphate dehydrogenase and ATP synthase, in the heart tissue of animals .A probability value of less than 0.05 was considered significant (P< 0.05). Conclusion: It seems that protein SUMOylation provides a safeguard mechanism against DZN Toxicity.
کلیدواژه‌های انگلیسی مقاله Diazinon, MALDI-TOF/TOF, SUMOlation, SUMO1
نویسندگان مقاله رضوان یزدیان رباطی | rezvan yazdian robati
department of biotechnology, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

آتنا پورتاجی | atena pourtaji
department of pharmacodynamy and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

مرضیه راشدی نیا | marzieh rashedinia
department of toxicology and pharmacology, school of pharmacy, international branch, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

حسین حسین زاده | hossein hosseinzadeh
pharmaceutical research center, department of pharmacology and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

مریم قربانی | maryam ghorbani
department of pharmacology and toxicology, school of pharmacy, baqiyatallah university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

بی بی مرجان رضوی | bibi marjan razavi
targeted drug delivery research center, department of pharmacology and toxicology, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

محمد رمضانی | mohammad ramezani
pharmaceutical research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

خلیل abnous | khalil abnous
pharmaceutical research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_6278.html
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