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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۱۰، صفحات ۱۰۲۰-۱۰۲۶
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عنوان انگلیسی MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
چکیده انگلیسی مقاله Objective(s):MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (CKD). In the present study, we elucidated the role of microRNA 205 (miR-205) in cisplatin-induced renal cell apoptosis and explored the molecular mechanisms. Materials and Methods:The chronic interstitial nephropathy rat model was induced, and the miRNA expression profile in the kidney cells from rats with CKD was screened. Cisplatin-induced apoptosis in normal renal HK-2 cells was evaluated using flow cytometry, and regulation of miR-205 on target gene was validated using luciferase assay, western blot and real time PCR assays. Results: We found that miR-205 expression was significantly decreased in the cells from kidney of CKD rat (P< 0.01). Our data showed that when miR-205 was overexpressed or silenced using the mimic or inhibitor, the percentages of apoptotic cells were suppressed or increased significantly (P< 0.05), respectively. Moreover, we have identified CMTM4 gene, which is involved in cell proliferation and apoptosis, as a novel target for miR-205. In addition, miR-205 could inhibit apoptosis by binding to the 3’UTR of CMTM4 mRNA and inhibiting its transcriptional activity. Conclusion: This study elucidated that miR-205 plays an important role in the regulation of apoptosis in renal cells, suggesting a potential therapeutic target to hinder CKD development.
کلیدواژه‌های انگلیسی مقاله Apoptosis, Chronic kidney disease, CMTM4, microRNA 205
نویسندگان مقاله hongxia زانگ | hongxia zhang
department of internal medicine, shengli oilfield central hospital, dongying city, shandong province, 257034, p. r. china


xiaoning زانگ | xiaoning zhang
department of internal medicine, shengli oilfield central hospital, dongying city, shandong province, 257034, p. r. china


xiaoying yuan | xiaoying yuan
department of internal medicine, shengli oilfield central hospital, dongying city, shandong province, 257034, p. r. china


linna ونگ | linna wang
department of internal medicine, shengli oilfield central hospital, dongying city, shandong province, 257034, p. r. china


ying محمد بن همام رییس کنفدراسیون | ying xiao
department of internal medicine, shengli oilfield central hospital, dongying city, shandong province, 257034, p. r. china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_5467.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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