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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۱۰، صفحات ۱۰۲۷-۱۰۳۳
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عنوان انگلیسی MiR-493 suppresses the proliferation and invasion of gastric cancer cells by targeting RhoC
چکیده انگلیسی مقاله Objective(s):MiRNAs have been proposed to be key regulators of tumorigenesis, progression and metastasis. However, their effect and prognostic value in gastric cancer is still poorly known. Materials and Methods: Gastric cancer cell lines were cultured. Tissue samples obtained from 36 gastric cancer patients were used for quantitative real-time PCR (qRT-PCR) analysis. The tissue microarrays (TMAs) consisted of 126 cases of gastric carcinoma that were used for In situ hybridisation (ISH). Lentivirus plasmids were co-transfected into 293FT cells. Cell migration was examined using wound-healing assays. Statistical analyses were performed using SPSS16.0 software. Results: In this study, we found that the expression levels of miR-493 were strongly down-regulated in gastric cancer and were associated with clinical stage and the presence of lymph node metastases. Moreover, miR-493 might independently predict OS and RFS in gastric cancer. We further found that up-regulation of miR-493 inhibited the proliferation and metastasis of gastric cancer cells, in vitro and in vivo. In addition, miR-493 directly targeted RhoC, which resulted in a marked reduction of the expression of mRNA and protein. This effect, in turn, led to a decreased ability of growth, invasion and metastasis in gastric cancer cells. Conclusion: Taken together, our findings demonstrate that miR-493 is important for gastric cancer initiation and progression and holds promise as a prognostic biomarker to predict survival and relapse in gastric cancer. It is also a potential therapeutic tool to improve clinical outcomes in this disease.
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نویسندگان مقاله wenhua zhou | wenhua zhou
cancer research institute, university of south china, key laboratory of cancer cellular and molecular pathology of hunan provincial university, hengyang, people amp;apos;s republic of china


chi زانگ | chi zhang
function laboratory center, medical school, university of south china, hengyang, hunan, people’s republic of china


hao jiang | hao jiang
center for gastric cancer research of human province, the first affiliated hospital, university of south china, hengyang, hunan, people amp;apos;s republic of china


zhiwei زانگ | zhiwei zhang
cancer research institute, university of south china, key laboratory of cancer cellular and molecular pathology of hunan provincial university, hengyang, people amp;apos;s republic of china


لیمینگ xie | liming xie
center for gastric cancer research of human province, the first affiliated hospital, university of south china, hengyang, hunan, people amp;apos;s republic of china


xiusheng he | xiusheng he
cancer research institute, university of south china, key laboratory of cancer cellular and molecular pathology of hunan provincial university, hengyang, people amp;apos;s republic of china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_5468.html
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نوع مقاله منتشر شده Original Article
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