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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۸، صفحات ۷۸۸-۷۹۸
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عنوان انگلیسی Decellularized kidney in the presence of chondroitin sulfate as a natural 3D scaffold for stem cells
چکیده انگلیسی مقاله Objective(s): Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, chondroitin sulfate sodium was used for impregnation of the scaffolds. It is a critical component in extracellular matrix and plays an important role in signaling pathway; however, little is known about its role within mammalian development and cell linage specification. Materials and Methods: Due to its porous and appropriate structure and for putting cells in 3D space, the kidney of BALB/c mouse was selected and decellulalized using physical and chemical methods. After decellularization, the scaffold was impregnated in chondroitin sulfate solution (CS) for 24 hr. Then, 60×10 5 human adipose-derived mesenchymal stem cells were seeded on the scaffold to assess their behavior on day 5, 10, 15, 20, and 25. Result: After 48 hr, DAPI staining approved completed decellularized kidney by 1% SDS (sodium dodecyl sulfate). Migration and establishment of a number of cells to the remaining area of the glomerulus was observed. In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure. Up to the day 15, microscopic study of different days of seeding showed the gradual adhesion of large number of cells to the scaffold. Conclusion: Glycosaminoglycan could be a right option for impregnation. It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.
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نویسندگان مقاله علیرضا رفیقدوست | alireza rafighdoust
department of biology, faculty of sciences, mashhad branch, islamic azad university, mashhad, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

ناصر مهدوی شهری | nasser mahdavi shahri
department of biology, faculty of sciences, ferdowsi university of mashhad, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

جواد بهارآرا | javad baharara
department of biology, faculty of sciences, mashhad branch, islamic azad university, mashhad, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_4730.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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