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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۶، صفحات ۵۴۴-۵۴۸
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عنوان انگلیسی Juglone exerts antitumor effect in ovarian cancer cells
چکیده انگلیسی مقاله Objective(s): Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells. Materials and Methods: MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-treated SKOV3 cells. To investigate molecular mechanism of cell cycle and apoptosis, protein expression levels were measured by Western blot analysis of cyclin D1, Bcl-2, Bax, cytochrome c, caspase-9 and caspase-3. To investigate the motility of juglone-treated SKOV3 cell, Matrigel invasion assay was employed to characterize cell invasion. Also, matrix metalloproteinase-2 (MMP-2) expression levels were detected by western blot. Results:Juglone significantly inhibited SKOV3 cell proliferation as shown by G0/G1 phase arrest, and this effect was mediated by inactivation of cyclin D1 protein (P< 0.05). Juglone induced apoptosis in SKOV3 cell which was accompanied by caspase-9 and caspase-3 activation (P< 0.05). Juglone decreased Bcl-2 levels and increased Bax and cytochrome c (Cyt c) levels(P< 0.05). Juglone sufficiently inhibited invasion while evidently decreased MMP-2 expression (P< 0.05). Conclusion:The results suggest that juglone could probably induce apoptosis through mitochondrial pathway and restrained cell invasiveness by decreasing MMP expression.
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نویسندگان مقاله fang fang | fang fang
department of immunology, jilin medical college, jilin, jilin, china


yingxin qin | yingxin qin
department of narcotization, affiliated hospital of jilin medical college, jilin, jilin, china


ling qi | ling qi
department of pathology, jilin medical college, jilin, jilin, china


qing fang | qing fang
department of immunology, jilin medical college, jilin, jilin, china


liangzhong zhao | liangzhong zhao
department of immunology, jilin medical college, jilin, jilin, china


shuang chen | shuang chen
department of immunology, jilin medical college, jilin, jilin, china


qiang li | qiang li
department of immunology, jilin medical college, jilin, jilin, china


duo زانگ | duo zhang
department of immunology, jilin medical college, jilin, jilin, china


liguo ونگ | liguo wang
department of urology surgery, affiliated hospital of jilin medical college, jilin, jilin, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_4525.html
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نوع مقاله منتشر شده Original Article
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