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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۴، صفحات ۳۵۰-۳۵۵
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عنوان انگلیسی Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis
چکیده انگلیسی مقاله Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. Materials and Methods: A chimeric gene encoding trigger factor (TF), Omp3148-74 and BP2687-111 fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coliBL21-DE3 and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits. Results: Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in 76.5% of sera obtained from infected patients. Conclusion: These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies.
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نویسندگان مقاله جعفر امانی | jafar amani
applied microbiology research center, baqiyatallah university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

امیر قاسمی | amir ghasemi
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

رضا رنجبر | reza ranjbar
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

مهدی شعبانی | mahdi shabani
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


مهدی zandemami | mahdi zandemami
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


رضا گل محمدی | reza golmohammadi
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_4283.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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