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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۳، صفحات ۲۹۳-۳۰۰
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عنوان انگلیسی Nerve growth factor in human semen: Effect of nerve growth factor on the normozoospermic men during cryopreservation process
چکیده انگلیسی مقاله Objective(s):Althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. Adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm DNA fragmentation and infertility. The post-thaw survival of spermatozoa improved after addition of supplementation of antioxidant molecules to freezing media. Nerve growth factor (NGF) as one of the prosurvival substances has gained great attention in recent years. The aim of this study was the usage of NGF as prosurvival factor after cryopreservation process of human semen samples to assess the motility and viability of sperm, nitric oxide (NO) concentration, and DNA fragmentation in normozoospermic men. Materials and Methods: Semen samples were collected from 25 normozoospermic men and were divided into fresh semen samples as control group, frozen–thawed semen samples without addition of exogenous NGF, and three groups of semen samples cryopreserved with addition of exogenous NGF (0.5, 1, and 5 ng/ml) in freezing medium. Viability was assessed by eosin-negrosin staining technique. Motility was evaluated with inverted microscope. NO concentration and apoptosis content were measured with flow cytometry. Results: Results showed that exogenous NGF at 0.5 ng/ml could significantly (P-value < 0.05) influence viability, motility, nitric oxide, and DNA fragmentation content. Conclusion: Exogenous NGF as cryoprotectant improved sperm viability and motility, increased intracellular NO concentration, and decreased apoptosis content in normal human spermatozoa.
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نویسندگان مقاله سارا سعیدنیا | sara saeednia
department of anatomy, school of medicine, baqiyatallah medical science university, tehran, iran


حسین بهادران | hosein bahadoran
department of anatomy, school of medicine, baqiyatallah medical science university, tehran, iran


فردین عمیدی | fardin amidi
department of anatomy, school of medicine, tehran medical science university, tehran, iran


محمد حسین اسدی | mohammad hosein asadi
department of anatomy, school of medicine, baqiyatallah medical science university, tehran, iran


محمد ناجی | mohammad naji
department of anatomy, school of medicine, tehran medical science university, tehran, iran. urology and nephrology research center unrc , shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

پروین فلاحی | parvin fallahi
department of infertility, shareati hospital, tehran, iran


ناهید عطایی نژاد | nahid ataie nejad
department of anatomy, school of medicine, tehran medical science university, tehran, iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_4134.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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