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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۲، صفحات ۱۰۸-۱۱۴
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی MicroRNAs that target RGS5
چکیده انگلیسی مقاله Objective(s):An earlier meta-analysis on gene expression data derived from four microarray, two cDNA library, and one SAGE experiment had identified RGS5 as one of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (TM) cells by all studies. RGS5 encodes regulator of G-protein signaling-5. The TM tissue is the route of aqueous fluid outflow, and is relevant to the pathology of glaucoma. MicroRNAs constitute the most recently identified components of the cellular machinery for gene regulation in eukaryotic cells. Given our long standing interest in glaucoma, we aimed to identify miRNAs that may target RGS5. Materials and Methods: Eight miRNAs were selected for study using bioinformatics tools and available data on miRNAs expressed in the eye. Their effects were assessed using the dual luciferase assay. 3'-UTR segments of RGS5 mRNA were cloned downstream of a luciferase coding gene in psiCHECK2 vectors, and these were co-transfected with each of the miRNAs into HEK293 cells. Results: The outcomes evidenced that one or more of the segments are in fact targeted by miR-7, miR-9, miR-96, miR-23a, miR-23b, miR-204, and miR-211. Down regulations by the miRNAs were statistically significant. The effect of miR-204 is considered particularly important as this miRNA is well known to regulate eye development and to affect multiple ocular functions. Conclusion: Our results justify further studies on regulation of RGS5 expression and RGS5 downstream functions by these miRNAs.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله امیر بنایی اصفهانی | amir banaei esfahani
department of biotechnology, college of science, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

حمیدرضا موذنی | hamidreza moazzeni
department of medical genetics, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

پویا ناصری نوسر | pooya naseri nosar
department of biotechnology, college of science, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

صدف امین | sadaf amin
department of biotechnology, college of science, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

احسان عارفیان | ehsan arefian
school of biology, college of science, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

مسعود سلیمانی | masoud soleimani
stem cell technology research center, tehran, iran


شاهین یزدانی | shahin yazdani
ophthalmic research center, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

الهه الهی | elahe elahi
department of biotechnology, college of science, university of tehran, tehran, iran. school of biology, college of science, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_4010.html
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زبان مقاله منتشر شده en
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