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Iranian Journal of Basic Medical Sciences، جلد ۱۸، شماره ۱، صفحات ۲۲-۲۹
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عنوان انگلیسی Human Wharton’s jelly-derived mesenchymal stem cells express oocyte developmental genes during co-culture with placental cells
چکیده انگلیسی مقاله Objective(s): The present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. Much more efforts have been made to develop a procedure for attaining germ cells in vitro. Recently, human umbilical cord-derived mesenchymal stem cells (HUMSCs) have been introduced with higher efficacy for differentiation. In this work, we tried to explore the efficacy of HUMSCs and some effective products of placental cells such as transforming growth factors. This study is aimed to optimize a co-culture condition for HUMSCs with placental cells to obtain primordial germ cells (PGCs) and reach into oocyte-like cells in vitro. Materials and Methods: In this experimental study, HUMSCs and placental cells were co-cultured for 14 days without any external inducer in vitro. Then HUMSCs were assessed for expression of PGC markers; Octamer-binding transcription factor 4(OCT4),Tyrosine-protein kinase Kit (CKIT), Stage specific embryonic antigen 4 (SSEA4) , DEAD (Asp-Glu-Ala-Asp) box polypeptide 4( DDX4) and oocyte specific markers; Growth differentiation factor-9( GDF9), Zona pellucida glycoprotein 3(ZP3). The pertinent markers were assessed by immunocytochemistry and Q-PCR. Results: Co-cultured HUMSCs with placental cells (including amniotic and chorionic cells) presented Oct4 and DDX4, primordial germ cells specific markers significantly, but increment in expression of oocyte-like cell specific markers, GDF9 and ZP3 did not reach to statistically significant threshold. Conclusion: Placental cell supplementsTransforming growth factor (TGF α, β) and basic fibroblast growth factor (bFGF) in a co-culture model can provide proper environment for induction of HUMSCs into PGCs and expression of oocyte-like markers.
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نویسندگان مقاله حمیدرضا عسگری | hamid reza asgari
department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد اکبری | mohammad akbari
department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی عباسی | mehdi abbasi
department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

جعفر ai | jafar ai
department of tissue engineering, school of advanced technologies, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مرتضی کروجی | morteza korouji
department of anatomy, faculty of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

فرشته علی اکبری | fereshte aliakbari
department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

kehinde adebayo babatunde | kehinde adebayo babatunde
department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فریدون سرگلزایی اول | fereydoon sargolzaei aval
department of anatomy, faculty of medicine, zahedan university of medical sciences, zahedan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زاهدان (Zahedan university of medical sciences)

محمد تقی جغتایی | mohammad taghi joghataei
department of anatomy, faculty of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_3883.html
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