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Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۱۱، صفحات ۹۱۲-۹۱۶
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چکیده فارسی مقاله
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عنوان انگلیسی Role of Brg1 in progression of esophageal squamous cell carcinoma
چکیده انگلیسی مقاله Objective(s): Epigenetic regulation of gene expression can be carried out through chromatin remodeling enzymes such as SWI/SNF. Brg1 also known as SMARCA4 is a catalytic subunit of SWI/SNF, which is necessary for MMPs expression. Matrix metalloproteinases (MMPs) are known as important player enzymes during tumor progression and metastasis. Aberrant epigenetic modification of chromatin should be precisely clarified to reveal probable unknown pathways in ESCC progression. Probable role of Brg1 in ESCC tumorigenesis and metastasis was studied through the assessment of Brg1 mRNA expression in KYSE30, and further evaluation about the biology of Brg1 was performed through the Brg1 silencing. Materials and Methods: Level of Brg1 mRNA expression in KYSE30 was compared to normal tissues using the real time polymerase chain reaction (PCR). Moreover, KYSE30 cells were transfected with Brg1-siRNA to silence the Brg1. Results: Our results showed for the first time that Brg1 mRNA expression was increased in KYSE30 cell line (ESCC cell line) compared with normal esophageal tissue of ESCC patients. Rate of transfection in KYSE30 was also between 40 to 50%, using the pSilencer-Brg1shRNA (1:1 ratio). Conclusion: Our data indicated that chromatin remodeling machinery is a novel aspect in tumor biology of ESCC, and overexpression of Brg1 as an important member of SWI/SNF might be involved in the migration and invasion of ESCC tumoral cells.
کلیدواژه‌های انگلیسی مقاله Brg1, ESCC, MMPs, Metastasis
نویسندگان مقاله شهرام ترکمندی | shahram torkamandi
medical genetics research center, medical school, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

میثم مقبلی | meysam moghbeli
division of human genetics, immunology research center, avicenna research institute, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

معین فرشچیان | moein farshchian
department of biology, faculty of science, ferdowsi university of mashhad, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

محمدرضا عباس زادگان | mohammad reza abbaszadegan
medical genetics research center, medical school, mashhad university of medical sciences, mashhad, iran 2 division of human genetics, immunology research center, avicenna research institute, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_3744.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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