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Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۹، صفحات ۶۴۶-۶۵۰
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عنوان انگلیسی Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method
چکیده انگلیسی مقاله Objective(s): The nosocomial infections by Aspergillus species are associated with constructions and increased dust loads in hospital indoors. Our main object was to find the environmental sources of Aspergillus species causing hospital acquired infections. Materials and Methods: The clinical and environmental samplings were performed during 18 months from spring 2010 to summer 2011 in Imam educational hospital, Urmia, Iran. A morphological diagnosis was performed including microscopic characterization of isolated aspergillus from cultured specimens and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) for the identification in the level of species. Random amplified polymorphic DNA – PCR RAPD-PCR using random primers for rDNA gene was performed to compare Aspergillus isolates of clinical cases with the relevant environmental sources. Results: Use of RAPD method resulted various differential patterns, so that some Aspergillus isolates from the clinical and hospital indoor were completely matched (matched pairs) and some other Aspergillus isolates were not matched. In the case of matched pairs, Aspergillus niger and A. flavus isolated from broncoalveolar lavage and sinus discharge were relevant to those of air conditioner and walls surfaces, respectively. Conclusion: The hospital sources for the Aspergillus clinical isolates included air condition and walls. RAPD-PCR analysis can play a trivial role to find the hospital sources of Aspergillus clinical isolates.
کلیدواژه‌های انگلیسی مقاله Aspergillus, Hospital, Molecular identification
نویسندگان مقاله کامبیز دیبا | kambiz diba
cellular and molecular research center, urmia university of medical sciences, urmia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

خدیجه مخدومی | khadijeh makhdoomi
imam educational hospital, urmia university of medical sciences, urmia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

حسین میرهندی | hossein mirhendi
esfahan institute of public health, tehran university of medical sciences, esfahan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_3323.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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