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Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۸، صفحات ۵۶۰-۵۶۵
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عنوان انگلیسی Immunoprotectivity of Salmonella enterica serovar Enteritidis virulence protein, InvH, against Salmonella typhi
چکیده انگلیسی مقاله Objective(s):Typhoid fever is a dreadful disease of a major threat to public health in developing countries. Vaccination with bacterial immunodominant components such as surface proteins may prove as a potent alternative to live attenuated vaccines. InvH, an important part of needle complex in type three secretion system (TTSS) plays important role in efficient bacterial adherence and entry into epithelial cells. Materials and Methods:In this work we used a 15 kDa recombinant InvH protein of Salmonella enteric serovar Enteritidis to provoke antibody production in mouse. The mice were immunized by recombinant InvH and challenged with Salmonella typhi. Histopathology of spleen and liver were studied. Results:The immunized mice showed a significant rise of antibody after the second booster. The immunization induced protection against high doses of S. typhi. The bacterial challenge with sera showed significant protection against challenge dose of 2×109 CFU. Immunized sera reacted with S. typhi markedly. Immunoreaction of bacterially infected sera and InvH protein was significantly higher than the control group. Bacterial loads of S. typhi in spleen was more than liver. Decreased bacterial load was evident in immunized mice after 7 days. Histological examination of the liver showed the immunized mice liver remained unaffected. Conclusion: Efficacy of the virulence protein, InvH, in inhibition of this phenomenon by active immunization was shown here. It may be concluded that InvH, as an antigen, can develop protection against S. typhi infections. InvH may be exploited in protective measures as well as a diagnostic tool in Salmonella infections.
کلیدواژه‌های انگلیسی مقاله Immunogenicity, InvH, Salmonella typhi, Vaccine
نویسندگان مقاله بهزاد دهقانی | behzad dehghani
department of biology, shahed university, tehran, iran

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

ایرج رسولی | iraj rasooli
department of biology, and molecular microbiology research center, shahed university, tehran, iran

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

محمدرضا jalali–nadoushan | mohammadreza jalali–nadoushan
department of pathology, school of medicine, shahed university, tehran, iran

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

پرویز اولیا | parviz owlia
department of microbiology, school of medicine, and molecular microbiology research center, shahed university, tehran, iran

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

زهره رسولی | zohreh rasooli
school of medicine, shahed university, tehran, iran

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_3186.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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