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Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۵، صفحات ۳۷۸-۳۸۳
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عنوان انگلیسی Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction
چکیده انگلیسی مقاله Objective(s):blaCTX-Mand blaPER are two genes that encode class A extended-spectrum β-lactamases (ESBLs) and can be responsible for therapeutic problems. This study was carried out to evaluate the molecular properties of these genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction (PCR), restriction digestion and sequencing. Materials and Methods: During six months, starting from January 2012, one hundred clinical isolates of Enterobacteriaceae were collected from urinary samples. The ESBL-producing isolates were detected by phenotypic confirmation test. After plasmid extraction, blaPER and blaCTX-M genes were detected using PCR by specific primers. The blaCTX-M PCR products were digested with Taq1, and two of the blaCTX-Mgenes were sequenced. Results: Phenotypic tests showed that 27 (27%) isolates were ESBL producers with the highest frequency for Klebsiella pneumoniae (47.4%) andEscherichia coli (17.9%). Twenty six (26%) of Enterobacteriaceae isolates harbored the blaCTX-Mgene, and none of them had blaPER. The restriction analysis of PCR products showed that all blaCTX-M amplified products had the same patterns. Both sequenced bacteria were CTX-M-15 type ESBL carriers. Conclusion: The results of this study showed the blaCTX-M-15gene in Enterobacteriaceae isolates for the first time in Mashhad, Iran. High degrees of associated resistance to co-trimoxazole and gentamicin were found in ESBL producers. Therefore, an integrated and regular management of antibiotic prescription need to be trained in our society.
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نویسندگان مقاله محبوبه نخعی مقدم | mahboobeh nakhaei moghaddam
department of biology, mashhad branch, islamic azad university, mashhad, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

مهرداد هاشمی بیدختی | mehrdad hashemi beidokhti
department of biology, science and research branch, islamic azad university, fars, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

سعید عامل jamehdar | saeid amel jamehdar
antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

martha قهرمان | martha ghahraman
medical genetic research center, school of medical science, mashhad, iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_2789.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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