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Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۲، صفحات ۸۱-۸۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Significance of microRNA targeted estrogen receptor in male fertility
چکیده انگلیسی مقاله Objective(s): Estrogen receptor-alpha (ERα) mediates estrogen action in regulation of different levels of the hypothalamic-pituitary-testis axis. It has a key role in spermatogenesis. Estrogen receptor alpha knock-out (ER koα) male mice were infertile and severe impairment in spermatogenesis and seminiferous tubules was observed. Recently, it has been reported that microRNA (miRNA) mir-100 and let-7b were predicted to target ERα gene. MiRNA are small, endogenous, single stranded RNA molecules that regulate gene expression and have been implicated in various disease states. It has been proved that some miRNAs expression is tissue- and disease-specific, giving potential for identifying miRNAs as a diagnostic tool. Materials and Methods: In this study, the change in the expression levels of mir-100, let-7b and ERα expression levels were evaluated in oligospermic infertile patients (n=43) compared to control fertile subjects (n=43). After washing and separating sperms, total RNA was isolated and then cDNA was synthesized. The expression levels of mir-100 and let-7b and ERα were evaluated by real time PCR. Results: Mir-100, let-7b levels were significantly higher than those in control group (P=0.008 and P=0.009, respectively). We have found that, ERα level was significantly decreased in comparison with normal group (P< 0.0001). Conclusion: Changes in mir-100, let-7b and ERα expression levels in oligospermic patients may be associated with the susceptibility and progression of infertility. The results of this study indicate that miRNA can have a key role in spermatogenesis and might have a diagnostic and prognostic value in men infertility.
کلیدواژه‌های انگلیسی مقاله Fertility, Has-mir-100, Has-let-7b, MicroRNA
نویسندگان مقاله علیرضا ابهری | alireza abhari
department of biochemistry and clinical laboratories, faculty of medicine, tabriz university of medical sciences, tabriz, iran women’s reproductive health research center, alzahra hospital, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نصرت اله ضرغامی | nosratollah zarghami
department of biochemistry and clinical laboratories, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

وحیده شهنازی | vahideh shahnazi
women’s reproductive health research center, alzahra hospital, tabriz, iran


ابوالفضل برزگر | abolfazl barzegar
research institute for fundamental sciences rifs , university of tabriz, tabriz, iran

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

لعیا فرزادی | laya farzadi
women’s reproductive health research center, alzahra hospital, tabriz, iran


هادی کرمی | hadi karami
department of biochemistry and clinical laboratories, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

سپیده zununi واحد | sepideh zununi vahed
department of biotechnology, faculty of advanced medical science, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

محمد نوری | mohammad nouri
women’s reproductive health research center, alzahra hospital, tabriz, iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_2238.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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