این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۱۷، شماره ۱، صفحات ۲۱-۲۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Phenotypic and genotypic characteristics of tetracycline resistant Acinetobacter baumannii isolates from nosocomial infections at Tehran hospitals
چکیده انگلیسی مقاله Objective(s):To date, the most important genes responsible for tetracycline resistance among Acinetobacter baumannii isolates have been identified as tet A and tet B. This study was carried out to determine the rate of resistance to tetracycline and related antibiotics, and mechanisms of resistance. Materials and Methods: During the years 2010 and 2011, a total of 100 A. baumannii isolates were recovered from patients in different hospitals of Tehran, Iran. Antimicrobial susceptibility to tetracycline, minocycline, doxicycline and tigecycline was evaluated by E-test. Polymerase chain reaction (PCR) of the tet A and tet B genes was performed using specific primers, after which the isolates were subjected to Repetitive Extragenic Palindromic-PCR (PCR) to identify the major genotypes. Results: Of all isolates, 89% were resistant to tetracycline (MIC50 = 32 μg/ml, MIC90 = 512 μg/ml). Minocycline with the resistant rate of 35% (MIC50 = 16 μg/ml, MIC90 =32 μg/ml) and doxicycline with the resistant rate of 25% (MIC50 = 16 μg/ml, MIC90= 32 μg/ml) have a good activity against A. baumannii isolates. All isolates were sensitive to tigecycline. Frequencies of tet B and tet A genes and coexistence of tet A and tet B among the isolates resistant to tetracycline, were 87.6%, 2.2% and 1.1%, respectively. Distribution of REP-types among A. baumannii isolates was types A (40%), B (30%), C (10%), D (5%) and E (5%). Conclusion: It seems that tet A and tet B genes play an important role in the induction of resistance towards tetracyclines used in this study. It is suggested that further studies focus on other antimicrobial drugs and combinations in order to achieve a successful therapy against multi drug resistance (MDR) A. baumannii strains in Iran.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله محمد حسین ملکی | mohammad hossein maleki
clinical microbiology research center, ilam university of medical sciences, ilam, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

zamberi sekawi | zamberi sekawi
department of medical microbiology and parasitology, faculty of medicine and health sciences, university of putra, malaysia


ستاره سروش | setareh soroush
clinical microbiology research center, ilam university of medical sciences, ilam, iran.department of microbiology, school of medicine, ilam university of medical sciences, ilam, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

فرید عزیزی جلیلیان | farid azizi jalilian
clinical microbiology research center, ilam university of medical sciences, ilam, iran 2 department of medical microbiology and parasitology, faculty of medicine and health sciences, university of putra, malaysia

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

خیرالله اسداللهی | khairollah asadollahi
psychosocial injuries research center, ilam university of medical sciences, ilam, ira

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

ستار محمدی | sattar mohammadi
department of microbiology, school of medicine, ilam university of medical sciences, ilam, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

محمد ایمان عینی | mohammad emaneini
department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مروت طاهری کلانی | morovat taherikalani
clinical microbiology research center, ilam university of medical sciences, ilam, iran 2 department of medical microbiology and parasitology, faculty of medicine and health sciences, university of putra, malaysia

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_2151.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات