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Iranian Journal of Basic Medical Sciences، جلد ۱۶، شماره ۱۰، صفحات ۱۱۱۴-۱۱۱۸
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عنوان انگلیسی Evaluation of the Relationship between the Incubation Time and Carotenoid Production in Rhodotorula Slooffiae and R. Mucilaginosa Isolated from Leather Tanning Wastewater
چکیده انگلیسی مقاله Objective(s): Carotenoids which are naturally synthesized by fungi such as yeasts can act as an antioxidant which is closely related to their ability to decrease the risk of a variety of degenerative diseases. In recent years, the increase of demand for carotenoids obtained from natural sources has promoted major efforts to improve carotenoid production from biological sources such as pigmented yeasts. The aim of this study was comparing incubation time and carotenoid production in Rhodotorula slooffiae and R. mucilaginosa isolated from leather tanning wastewater. Materials and Methods: To isolate the carotenoid pigment, cells were suspended in acetone and broken using a homogenizer, followed by centrifugation and separation of supernatant. In order to study the effect of incubation time, samples were held at 30 С in a shaker at 150 rpm for 24, 48, 72, 96, and 120 hr. For analytical evaluation, pigments were measured spectrophotometrically at 450 nm using the extinction coefficient E1%450=2500. Results: The results showed that the content of total carotenoid in R. slooffiae was the highest when samples were incubated for 72 hr. Overall, R. mucilaginosa had more potential to produce carotenoid. The best incubation periods for R. slooffiae and R. mucilaginosa were 72 hr and 48 hr, respectively. Conclusion: It seemed that the maximum rate of total carotenoid was not directly associated with the maximum amount of cell biomass and the type of carotenoid and their relative amount may vary depending on genus of yeast.
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نویسندگان مقاله فرزانه سادات نقوی | farzaneh sadat naghavi
department of biology, faculty of science, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

پریچهر حناچی | parichehr hanachi
department of biology, faculty of science, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

محمدرضا صعودی | mohammad reza soudi
department of biology, faculty of science, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

عذرا صبورا | azra saboora
department of biology, faculty of science, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

عاطفه قربانی | atefeh ghorbani
department of biology, faculty of science, alzahra university, tehran, iran

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_1875.html
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Short Communications
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