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Iranian Journal of Basic Medical Sciences، جلد ۱۶، شماره ۶، صفحات ۷۶۳-۷۷۱
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عنوان انگلیسی Comparison between Chondrogenic Markers of Differentiated Chondrocytes from Adipose Derived Stem Cells and Articular Chondrocytes In Vitro
چکیده انگلیسی مقاله Objective(s): Osteoarthritis is one of the most common diseases in middle-aged population in the world. Cartilage tissue engineering (TE) has been presented as an effort to introduce the best combination of cells, biomaterial scaffolds and stimulating growth factors to produce a cartilage tissue similar to the natural articular cartilage. In this study, the chondrogenic potential of adipose derived stem cells (ADSCs) was compared with natural articular chondrocytes cultured in alginate scaffold. Materials and Methods: Human ADSCs were obtained from subcutaneous adipose tissue and human articular chondrocytes from non-weight bearing areas of knee joints. Cells were seeded in 1.5% alginate and cultured in chondrogenic media for three weeks with and without TGFβ3. The genes expression of types II and X collagens was assessed by Real Time PCR and the amount of aggrecan (AGC) and type I collagen measured by ELISA and the content of glycosaminoglycan evaluated by GAG assay. Results: Our findings showed that type II collagen, GAG and AGC were expressed, in differentiated ADSCs. Meanwhile, they produced a lesser amount of types II and X collagens but more AGC, GAG and type I collagen in comparison with natural chondrocytes (NCs). Conclusion: Further attempt should be carried out to optimize achieving type II collagen in DCs, as much as, natural articular chondrocytes and decline of the production of type I collagen in order to provide efficient hyaline cartilage after chondrogenic induction, prior to the usage of harvested tissues in clinical trials.
کلیدواژه‌های انگلیسی مقاله Alginate Stem cell Chondrocyte TGFbeta
نویسندگان مقاله محمد مردانی | mohmmad mardani
department of anatomical sciences and molecular biology, medical school, esfahan university of medical sciences, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

بتول هاشمی بنی | batool hashemibeni
department of anatomical sciences and molecular biology, medical school, esfahan university of medical sciences, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

ملک مسعود انصار | malek masoud ansar
department of anatomical sciences and molecular biology, medical school, esfahan university of medical sciences, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

سید حمید زرکش اصفهانی | sayeed hamid zarkesh esfahani
department of immunology, medical school, esfahan university of medical sciences, esfahan, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

محمد کاظمی | mohmmad kazemi
department of anatomical sciences and molecular biology, medical school, esfahan university of medical sciences, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

وحید گوهریان | vahid goharian
amin hospital, medical school, esfahan university of medical sciences, esfahan, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

نفیسه اسماعیلی | nafiseh esmaeili
department of immunology, medical school, esfahan university of medical sciences, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

ابراهیم اسفندیاری | ebrahim esfandiary
department of anatomical sciences and molecular biology, medical school, esfahan university of medical sciences, esfahan, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_992.html
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