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Iranian Journal of Basic Medical Sciences، جلد ۱۵، شماره ۶، صفحات ۱۱۸۵-۱۱۹۰
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عنوان انگلیسی In vitro Assay of Human Gingival Scaffold in Differentiation of Rat’s Bone Marrow Mesenchymal Stem Cells to Keratinocystes
چکیده انگلیسی مقاله Objective(s)Tissue engineering is an attractive science because it promises new therapeutic strategies for repairing organs that have lost functions due to damage. The purpose of this study was to evaluate induction effect of human gingival scaffold in tissue engineering for skin regeneration.Materials and MethodsTissue samples were obtained from crown-lengthening procedures and wisdom teeth removal. The samples were decellularized and used as a scaffold for loading of rat BM-MSCs. The human gingival scaffolds loaded by bone marrow mesenchymal stem cells were derived from Wistar rat. Finally, it was evaluated via electron micrographs, as well as immunohistochemical techniques at day 7, 14, and 28 after initial seeding.ResultsThe histologic sections of human gingival scaffold –loaded rat BM-MSCs demonstrated formation of epithelial like layers at days 7, 14 and 28 after initial seeding. Scanning electron microscope (SEM) of the scaffolds indicated formed desmosomal adhesions, which revealed a degree of differentiation toward keratinocytes. The results of immunohistochemical staining were strongly positive for multi cytokeratin (CK) 14 days after initial seeding in epithelial differentiation. Rat BM-MSCs which loaded on human gingival scaffold is capable of differentiating toward keratinocytes.ConclusionGingival tissues were presented as a natural scaffold for attachment and differentiation of bone marrow mesenchymal stem cells towards keratinocytes, and might be used as suitable scaffold for reconstruction of the skin.
کلیدواژه‌های انگلیسی مقاله Acellular gingiva, Keratinocytes, Tissue engineering
نویسندگان مقاله ناصر مهدوی شهری | nasser mahdavishahri
department of biology, faculty of sciences, ferdowsi university of mashhad, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

مریم moghatam متین | maryam moghatam matin
department of biology, faculty of sciences, ferdowsi university of mashhad, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

مسعود فریدونی | masoud fereidoni
department of biology, faculty of sciences, ferdowsi university of mashhad, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

زهرا یارجانلی | zahra yarjanli
department of biology, faculty of sciences, ferdowsi university of mashhad, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

سید علی بنی هاشم راد | seyed ali banihashem rad
dental research centre, faculty of dentistry, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

سعیده خواجه احمدی | saeedeh khajeh ahmadi
oral and maxillofacial diseases research centre, faculty of dentistry, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_4939.html
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زبان مقاله منتشر شده en
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