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Iranian Journal of Basic Medical Sciences، جلد ۱۴، شماره ۱، صفحات ۱۷-۲۴
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عنوان انگلیسی Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion
چکیده انگلیسی مقاله Objective (s) Porin is a mitochondrial outer membrane channel, which usually functions as the pathway for the movement of various substances in and out of the mitochondria and is considered to be a component of the permeability transition (PT) pore complex that plays a role in the PT. We addressed the hypothesis that porin interacts with other mitochondrial proteins after ischemic injury. Materials and Methods For this purpose, we used in vivo 4-vessel occlusion model of rat brain and porin purification method by hydroxyapatite column. After SDS gel electrophoresis and silver nitrate staining, Western blotting was done for porin, adenine nucleotide translocase and cyclophilin-D proteins. Results Porin was purified from mitochondrial mixture in ischemic brain and control groups. Investigation of interaction of adenine nucleotide transposes (ANT) and cyclophilin-D with porin by Western blotting showed no proteins co-purified with porin from injured tissues. Conclusion The present study implies that there may not be interaction between porin, and ANT or cyclophilin-D, and if there is any, it is not maintained during the purification procedure.
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نویسندگان مقاله محمد علی اطلسی | mohammad ali atlasi
anatomical sciences research center, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

خوزه لوییس perez velazquez | jose luis perez velazquez
department of neurology and pediatrics, the hospital for sick children, brain and behavior programme, university of toronto, ontario, canada
نشانی اینترنتی http://ijbms.mums.ac.ir/article_4951.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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