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Iranian Journal of Basic Medical Sciences، جلد ۱۳، شماره ۳، صفحات ۶۹-۷۵
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عنوان انگلیسی Detection of Neuraminidase Activity in Pseudomonas aeruginosa PAO1
چکیده انگلیسی مقاله Objective(s) Some properties of neuraminidase produced by Pseudomonas aeruginosa PAO1 growth in a defined medium (BHI) were examined and evaluated for its features. Materials and Methods The obtained supernatant enzyme of P. aeruginosa PAO1 cultures was used in a sensitive fluorometric assay by using 2'-(4-methylumbelliferyl) a-D-N acetylneuraminic acid as substrate. As hydrolyzing MUN with neuraminidase; free N-acetylneuraminic acid and 4-methylumbelliferone were formed with a shift in the fluorescence spectra from 315/374 nm (substrate) to 365/450 nm (product). Enzyme activity was then measured by the fluorescence of 4-methylumbelliferone at 450 nm. Results Among the culture media to determine the enzyme production, the highest production of P. aeruginosa PAO 1 neuraminidase was found in BHI culture media. Neuraminidase production in P. aeruginosa PAO1 paralleled bacterial growth in defined medium (BHI) and was maximal in the late logarithmic phase of growth but decreased during the stationary phase, probably due to protease production or thermal instability. The neuraminidase of P. aeruginosa PAO1 possessed an optimum temperature of 56 °C and the activity was pH-dependent with maximal activity at pH 5. Heating the enzyme at 56 °C for 45 min in the presence of bovine serum albumin destroyed 33.1% of the activity while the addition of Ca+2, EDTA and N-acetyl neuraminic acid (NANA) decreased activity markedly. Conclusion Overall, the results indicated that neuraminidase of P. aeruginosa PAO1 is more an extracellular enzyme than K. pneumonia neuraminidase is.
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نویسندگان مقاله سیامک قضایی | ciamak ghazaei
department of microbiology, faculty of veterinary, university of urmia, urmia, iran

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

ملاحت احمدی | malahat ahmadi
department of microbiology, faculty of veterinary medicine, university of urmia, urmia, iran

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

نیما حسینی جزنی | nima hosseini jazani
department of microbiology, immunology and genetics, faculty of medicine, urmia university of medical sciences, uremia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_5087.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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