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Iranian Journal of Basic Medical Sciences، جلد ۱۳، شماره ۳، صفحات ۱۳۹-۱۴۵
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عنوان انگلیسی High Frequency Electromagnetic Field Induces Lipocalin 2 Expression in Mouse Liver
چکیده انگلیسی مقاله Objective(s) Neutrophil gelatinase-associated lipocalin (NGAL/Lcn2), comprise a group of small extracellular proteins with a common P-sheet-dominated 3-dimensional structure. In the past, it was assumed that the predominant role of lipocalin was acting as transport proteins. Recently it has been found that oxidative stress induces Lcn2 expression. It has been also proved that electromagnetic field (EMF) produces reactive oxygen species (ROS) in different tissues. Expression of Lcn2 following exposure to electromagnetic field has been investigated in this study. Materials and Methods Balb/c mice (8 weeks old) were exposed to 3 mT, 50 HZ EMF for 2 months, 4 hr/day. Afterwards, the mice were sacrificed by cervical dislocation and livers were removed. The liver specimens were stained with Haematoxylin- Eosin (H&E) and analyzed under an optical microscope. Total RNA was extracted from liver and reverse transcription was performed by Superscript III reverse transcriptase with 1 pg of total RNA. Assessment of Lcn2 expression was performed by semiquantitative and real time- PCR. Results The light microscopic studies revealed that the number of lymphocyte cells was increased compared to control and dilation of sinosoids was observed in the liver. Lcn2 was up-regulated in the mice exposed to EMF both in mRNA and protein levels. Conclusion To the extent of our knowledge, this is the first report dealing with up-regulation of Lcn2 in liver after exposure to EMF. The up-regulation might be a compensatory response that involves cell defense pathways and protective effects against ROS. However, further and complementary studies are required in this regards.
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نویسندگان مقاله آمنه محمدی روشنده | amaneh mohammadi roushandeh
anatomical sciences department, medicine faculty, medical university of tabriz, tabriz, iran

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

راحله حلبیان | raheleh halabian
research center, iranian blood transfusion organization ibto , tehran, iran|biotechnology department, tarbiyat modares uiversity, tehran, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

جعفر سلیمانی راد | jafar soleimani rad
anatomical sciences department, medicine faculty, medical university of tabriz, tabriz, iran

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

بهناز صادق زاده اسکویی | behnaz sadeghzadeh oskouei
anatomical sciences department, medicine faculty, medical university of tabriz, tabriz, iran

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

احمد مهدی پور | ahmad mehdipour
anatomical sciences department, medicine faculty, medical university of tabriz, tabriz, iran

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

محمدرضا نورانی | mohammad reza nourani
chemical injury research center, baqiyatallah medical science university, tehran iran


مهریار حبیبی رودکنار | mehryar habibi roudkenar
research center, iranian blood transfusion organization ibto , tehran, iran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_5103.html
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