این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۱۲، شماره ۲، صفحات ۷۰-۷۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Ex vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow
چکیده انگلیسی مقاله Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then differentiated among the osteogenic, adipogenic and chondrogenic cell lineages to determine their MSC nature. Differentiations were determined by RT-PCR analysis of related gene expression. To identify the best culture conditions for propagation, passage-3 cells were plated either at varying cell densities or different fetal bovine serum (FBS) concentrations for a week, at the end of which the cultures were statistically compared with respect to the cell proliferation. In this study, we also determined goat MSC population doubling time (PDT) as the index of their in vitro expansion rate. Results Passage-3 fibroblastic cells tended to differentiate into skeletal cell lineages. This was evident in both specific staining as well as the specific gene expression profile. Moreover, there appeared to be more expansion when the cultures were initiated at 100 cells/cm[1] in a medium supplemented with 15% FBS. A relatively short PDT (24.94±2.67 hr) was a reflection of the goat MSC rapid rate of expansion. Conclusion Taken together, fibroblastic cells developed at goat marrow cell culture are able to differentiate into skeletal cell lineages. They undergo extensive proliferation when being plated at low cell density in 15% FBS concentration.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله محمدرضا باغبان اسلامی نژاد | mohamadreza baghaban eslaminejad
stem cell department, cell science research centre, royan institute, acecr, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

حمید نظریان | hamid nazarian
stem cell department, cell science research centre, royan institute, acecr, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

فهیمه فلاحی | fahimeh falahi
stem cell department, cell science research centre, royan institute, acecr, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

لیلا تقی یار | leila taghiyar
stem cell department, cell science research centre, royan institute, acecr, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

محمد تقی دانش زاده | mohamad taghi daneshzadeh
royan animal facility, karaj, iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_5146.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات