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فصلنامه علمی پژوهشی گیاهان دارویی، جلد ۳، شماره ۴۷، صفحات ۱۰۰-۱۱۰
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عنوان انگلیسی Over - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.)
چکیده انگلیسی مقاله Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production. Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis. Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively. Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture.
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نویسندگان مقاله a قادری | a qaderi
department of plant breeding, science and research branch, islamic azad university

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

m امیدی | m omidi
department of agronomy and plant breeding, faculty of agriculture, tehran university

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

ar زبرجدی | ar zebarjadi
agronomy and plant breeding department, faculty of agriculture, razi university

سازمان اصلی تایید شده: دانشگاه رازی (Razi university)

r حاجی آقایی | r hajiaghaee
pharmacognosy amp;amp; pharmaceutics department of medicinal plants research center, institute of medicinal plants, acecr

سازمان اصلی تایید شده: پژوهشکده‮ ‬گیاهان‮ ‬دارویی‮ ‬جهاد‮ ‬دانشگاهی (Institute of medicinal plants)

نشانی اینترنتی http://www.jmp.ir/browse.php?a_code=A-10-75-2&slc_lang=en&sid=fa
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده بیوتکنولوژی
نوع مقاله منتشر شده پژوهشی
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