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فصلنامه علمی پژوهشی گیاهان دارویی، جلد ۱، شماره ۴۹، صفحات ۷۵-۸۶

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عنوان انگلیسی Seed Alkaloids Content and Antioxidant Enzymes Activity in Black Henbane as Influenced by Ammonium Nitrate Application and Water Deficit Stress
چکیده انگلیسی مقاله Background: Since alkaloids are nitrogenous compounds, the availability of nitrogen (N) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. Objective: This study intended to investigate the nitrogen (N) fertilization and water deficit stress (WDS) effects on seed tropane alkaloids elicitation including hyoscyamine (HYO) and scopolamine (SCO), and also antioxidant enzymes activities variations including superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) in black henbane. Methods: Plants were treated with different nitrogen (0, 0.14, 0.28 and 0.56 g N pot-1 as ammonium nitrate, N0-N3, respectively) and WDS treatments (30, 60 and 90% depletion of water from field capacity, W1-W3). Alkaloids extracted were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis. Results: Results showed that the highest alkaloid content values in seeds (HYO: 0.145% dw and SCO: 0.271% dw) achieved in plants grown under sever (W3) and moderate (W2) WDS accompanied with nitrogen supply of 0.28 g N pot-1 (N2), respectively. The maximum and minimum (2.112 and 0.114 g.plant-1) total alkaloids yield were obtained in N3W1 and N3W3 treatments, respectively. Furthermore, SOD activity increased with increasing N fertilization in all WDS treatments. CAT activity increased with WDS up to 60% FC, and then decreased with WDS increase. The POX activity was the opposite to that of CAT activity with N application under WDS levels. Conclusion: Our results suggest that N in appropriate level may act as a modifier for seed physiological responses and as an elicitor for tropane alkaloids biosynthesis pathway in black henbane (hyoscyamus niger) plants.
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نویسندگان مقاله m قربانپور | m ghorbanpour
department of medicinal plants, faculty of agriculture and natural resources, arak university, arak, 38156-8-8349, iran tel 98- 911- 3927299, fax 98-861-2761007

سازمان اصلی تایید شده: دانشگاه اراک (Arak university)

r غفارزادگان | r ghafarzadegan
research center, institute of medicinal plants, acecr

سازمان اصلی تایید شده: پژوهشکده‮ ‬گیاهان‮ ‬دارویی‮ ‬جهاد‮ ‬دانشگاهی (Institute of medicinal plants)

m حاتمی | m hatami
arak university

سازمان اصلی تایید شده: دانشگاه اراک (Arak university)


نشانی اینترنتی http://www.jmp.ir/browse.php?a_code=A-10-69-1&slc_lang=en&sid=fa
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده گیاهان دارویی
نوع مقاله منتشر شده پژوهشی
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