| چکیده انگلیسی مقاله |
Background and Objectives: Adhesion and colonization are prerequisites for the establishment of bacterial pathogenesis. The biofilm development of Pseudomonas aeruginosa was assessed on adhesive surfaces like dialysis membrane, stainless steel, glass and polystyrene. Materials and Methods: Microtiter plate biofilm assay was performed to assess the effect of nutrient medium and growth parameters of P. aeruginosa. Further, its growth on adhesive surfaces namely hydrophilic (dialysis membrane) and hydrophobic (polystyrene plate, square glass and stainless steel coupon) was assessed. The exopolysaccharide (EPS) was quantified using ruthenium red microplate assay and microscopic analysis was used to observe P. aeruginosa biofilm architecture. The anti-biofilm activity of herbal extracts on mature P. aeruginosa was performed. Results: The formation of large scale biofilms on dialysis membrane for 72 h was proved to be the best surface. In microscopic studies, very few exopolysaccaride fibrils, indicating a rather loose matrix was observed at 48 h. Further, thick exopolysaccaride, indicated higher adhesive properties at 72 h which is evident from ruthenium red staining. Among the plant extract used, Justicia wynaadensis leaf and Aristolochia indica (Eswari) root extract showed significant reduction of anti-biofilm activity of 0.178 OD and 0.192 OD in inhibiting mature biofilms at 0.225 OD respectively, suggesting the possible use of these extracts as efficient anti-adhesive and biofilm-disrupting agents with potential applications in controlling biofilms on surfaces. Conclusion: Our study facilitates better understanding in the development of P. aeruginosa biofilms on different food processing and clinical surfaces ultimately taking care of food safety and hygiene. |
| نویسندگان مقاله |
فرحان zameer | farhan zameer
mahajana life science research centre, department of studies in biotechnology and microbiology, pooja bhagavat memorial mahajana post graduate wing of sbrr mahajana first grade college, affiliated to university of mysore, k.r.s. road, metagalli, mysore - 570 016, karnataka, india.
rukmangada ms | rukmangada ms
mahajana life science research centre, department of studies in biotechnology and microbiology, pooja bhagavat memorial mahajana post graduate wing of sbrr mahajana first grade college, affiliated to university of mysore, k.r.s. road, metagalli, mysore - 570 016, karnataka, india.
jyoti بالا chauhan | jyoti bala chauhan
mahajana life science research centre, department of studies in biotechnology and microbiology, pooja bhagavat memorial mahajana post graduate wing of sbrr mahajana first grade college, affiliated to university of mysore, k.r.s. road, metagalli, mysore - 570 016, karnataka, india.
shaukath آرا khanum | shaukath ara khanum
department of chemistry, yuvaraja’s college constituent college , university of mysore, mysore - 570 005, karnataka, india.
pramod کومار | pramod kumar
mahajana life science research centre, department of studies in biotechnology and microbiology, pooja bhagavat memorial mahajana post graduate wing of sbrr mahajana first grade college, affiliated to university of mysore, k.r.s. road, metagalli, mysore - 570 016, karnataka, india.
aishwarya tripurasundari devi | aishwarya tripurasundari devi
department of biotechnology, sri jayachamarajendra college of engineering, mysore - 570 006, karnataka, india.
nagendra prasad mn | nagendra prasad mn
department of biotechnology, sri jayachamarajendra college of engineering, mysore - 570 006, karnataka, india.
dhananjaya bl | dhananjaya bl
toxinology toxicology and drug discovery unit, center for emerging technologies, jain global campus, jain university, kanakapura taluk, bangalore-562 112, karnataka, india.
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