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Iranian Journal of Microbiology، جلد ۸، شماره ۱، صفحات ۱-۷
عنوان فارسی
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عنوان انگلیسی A bioassay-guided fractionation scheme for characterization of new anti- bacterial compounds from Prosopis cineraria aerial parts
چکیده انگلیسی مقاله Background and Objectives: Due to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cineraria aerial parts were investigated using a bioassay guided fractionation scheme. Materials and Methods: The organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary characterization and identification of bioactive substance(s) was based on a bio-autograph- ical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS. Results: The best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 µg/ml synergistically. Conclusion: Further analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents.
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نویسندگان مقاله مهدیه نقابی حاجی آقا | mahdieh neghabi hajiagha
department of microbiology, school of medicine, tehran university of medical sciences, poorsina street, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

آتوسا علی احمدی | atousa aliahmadi
department of biology, medicinal plants and drugs research institute, shahid beheshti university.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

محمد رضا طاهری | mohammad reza taheri
department of phytochemistry, medicinal plants and drug research institute, shahid beheshti university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

علیرضا قاسمپور | alireza ghassempour
department of phytochemistry, medicinal plants and drug research institute, shahid beheshti university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

غلامرضا ایراجیان | gholamreza irajian
department of microbiology, school of medicine, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

حسن رضادوست | hassan rezadoost
department of phytochemistry, medicinal plants and drug research institute, shahid beheshti university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

محمدمهدی فیض آبادی | mohammadmehdi feizabadi
department of microbiology, school of medicine, tehran university of medical sciences, poorsina street, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/901
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