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Iranian Journal of Microbiology، جلد ۷، شماره ۶، صفحات ۳۱۹-۳۲۳
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Rapid fluorometric quantification of bacterial cells using Redsafe nucleic acid stain
چکیده انگلیسی مقاله Background and Objectives: Numerous procedures in biology and medicine require the counting of cells. Direct enumer- ation of Colony Forming Units (CFUs) is time-consuming and dreary accurate cell counting on plates with high numbers of CFUs is error prone. In this study we report a new indirect cell counting method that was developed based on the use of Redsafe fluorometric assay. The usefulness of Redsafe, a nucleic acid stain, in liquid medium is based on the binding of the fluorescent dye to DNA. Materials and Methods: Redsafe fluorometric assay was evaluated in comparison with MTT colorimetric assay as a colou- rimetric assay for enumeration of bacterial cells. Results: Obtained results showed that fluorometric assay threshold for LB grown E. coli is 6×104 CFU/ml. Redsafe fluo- rescent assay can be used as a rapid and inexpensive method for bacterial enumeration and quantification with increased sensitivity. Conclusion: The sensitivity of the Redsafe fluorometric assay for detection and enumeration of bacterial cells was 2-log-unit more than that was observed for the MTT assay.
کلیدواژه‌های انگلیسی مقاله Fluorometric assay,Redsafe,MTT assay,E.coli
نویسندگان مقاله احسان خلیلی | ehsan khalili
department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

وحید حسینی | vahid hosseini
department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رویا صلحی | roya solhi
department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مهدی امینیان | mahdi aminian
department of clinical biochemistry, school of medicine, tehran university of medical sciences, tehran, iran and recombinant vaccine research center, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/808
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زبان مقاله منتشر شده en
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