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Iranian Journal of Microbiology، جلد ۷، شماره ۴، صفحات ۱۸۵-۱۹۰
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عنوان انگلیسی Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus
چکیده انگلیسی مقاله Background and Objectives: Loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. In this study, a LAMP assay was established for Yersinia enterocolitica , a leading cause of acute enterocolitis in young children. Materials and Methods: LAMP assay was established with four primers targeting a specific locus for the detection of Y. enterocolitica . The assay was conducted at 65°C in thermo block for 90min. The sensitivity of LAMP was evaluated in com- parison to conventional PCR using pTZ57R containing the target locus. Finally, specificity was assessed using DNA from common enteropathogenic bacteria. Results: Results showed that the sensitivity of LAMP assay was 44-copy number, which was 10-fold higher than that of PCR. No cross-reactivity was observed when testing against other enteropathogenic pathogens. Conclusion: This study showed that LAMP assay is an alternative molecular diagnostic tool for infections with Y. enteroco- litica . In addition, this method may be useful in diagnosis at field or in laboratories without PCR machine.
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نویسندگان مقاله رضا رنجبر | reza ranjbar
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

داود افشار | davoud afshar
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/784
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