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Iranian Journal of Microbiology، جلد ۶، شماره ۳، صفحات ۱۸۴-۱۸۹
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عنوان انگلیسی Evaluation of New ELISA based on rLsa63 - rLipL32 antigens for serodiagnosis of Human Leptospirosis.
چکیده انگلیسی مقاله Background and objectives: Timely diagnosis of leptospirosis is essential for an effective treatment. Large diversity of clinical symptoms has led leptospirosis diagnosis difficult. Researchers have conducted many tests with wide-range of sensitivity and specificity to achieve novel diagnostic procedures which have higher sensitivity and specificity compared with previous tests and which are more reliable and available to public laboratories. This study aimed to introduce Lsa63 and LipL32 proteins-based ELISA tests with more sensitivity, specificity, accuracy and convenience for public laboratories. Materials and Methods: Recombinant forms of Lsa63 and LipL32 proteins were first generated. After coating these proteins, IgM and IgG ELISA tests were performed. 220 patients with suspicion of leptospirosis infection were selected for serum collection. The sera tests were carried out using MAT, IgM and IgG ELISA tests. In order to assess the performance of ELISA, the results of this test were compared with MAT. Results: 30% of serum samples (n=65) in MAT were positive for leptospirosis infection, while ELISA tests including rLipL32- rLsa63-IgM and rLipL32-rLsa63-IgG showed 40.45% (n=89) and 38.63% (n=80) positive reaction respectively. Conclusion: Our results demonstrated that new ELISA tests based on mixing LipL32 and Lsa63 proteins, a novel mixture of recombinant antigens, are valuable to detect specific antibodies against pathogenic Leptospira in human serum and could be considered as helpful techniques in leptospirosis diagnosis.
کلیدواژه‌های انگلیسی مقاله ELISA,Leptospira,LipL32 protein,Lsa63 protein,leptospirosis
نویسندگان مقاله صفرعلی علیزاده | safarali alizadeh
department of pathobiology, school of public health and institute of public health research, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

غلامرضا عبداله پور | gholamreza abdolahpour
faculty of veterinary medicine, university of tehran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمدرضا پورمند | mohammadreza pourmand
department of pathobiology, school of public health and institute of public health research, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

تقی ناصرپور | taghi naserpour
department of microbiology, qazvin university of medical sciences, qazvin, iran and cell and molecular research center, qazvin university of medical sciences, qazvin, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

رضا نجفی پور | reza najafipour
cell and molecular research center, qazvin university of medical sciences, qazvin, iran and department of biochemistry, qazvin university of medical sciences, qazvin, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

سید سعید اشراقی | seyyedsaeed eshraghi
department of pathobiology, school of public health and institute of public health research, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/417
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