این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۶، شماره ۴، صفحات ۲۳۰-۲۳۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Resistance of nanobacteria isolated from urinary and kidney stones to broad-spectrum antibiotics.
چکیده انگلیسی مقاله Background and Objective: Nanoscopic life forms called Nanobacteria or calcifying nanoparticles (CNP) are unconventional agents. These novel organisms are very small (0.1 to 0.5 microns) and possess unusual properties such as high resistance to heat and routine antimicrobial agents. Nanobacteria are 100 times smaller than bacteria and protected by a shell of apatite, so they could be as candidate for emerging and progress of in vivo pathological calcification. In this study, the inhibitory effect of broad-spectrum antibiotics on growth of these new forms of life has been investigated. Material and Methods: Powdered urinary and kidney stones were demineralized with HCl and neutralized with appropriate buffers and became filtered. Finally suspension was incubated in DMEM medium with Fetal Bovine Serum (FBS) and broad-spectrum antibiotics (100U/ml for penicillin and 100µg/ml for streptomycin) for 60 days. Results: In the presence of broad-spectrum antibiotics, Scanning Electron Micrographs (SEM) showed a spherical shape of these nanobacteria. Also, Energy Dispersive X-ray spectroscopy (EDS) showed a pick for calcium and phosphor. Transmission Electron Microscopy (TEM) results illustrated cover around the nanobacteria. Conclusion: The growth of calcifying nanoparticles after adding the broad-spectrum antibiotics may be due to their apatite hard shells supporting them against penetration of the antibiotics.
کلیدواژه‌های انگلیسی مقاله Broad-spectrum antibiotics,Nanobacteria,kidney stones
نویسندگان مقاله هادی سردارآبادی | hadi sardarabadi
department of biology, faculty of science, ferdowsi university of mashhad, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

منصور مشرقی | mansour mashreghi
department of biology, faculty of science, ferdowsi university of mashhad, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

خدیجه جامی الاحمدی | khadijeh jamialahmadi
biotechnology research center, mashhad university of medical sciences, mashhad, iran and yazd university, yazd, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

طاهره دیانت | tahere dianat
department of biology, faculty of science, ferdowsi university of mashhad, mashhad, iran.

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/403
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