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Iranian Journal of Microbiology، جلد ۶، شماره ۴، صفحات ۲۴۶-۲۵۲
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عنوان انگلیسی Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene.
چکیده انگلیسی مقاله Background and Objectives: To investigate coagulase gene polymorphisms of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. Materials and Methods: A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP. Results : There were 27 and 28 different RFLP patterns for Alu I and Hae III restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme. Conclusion : PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.
کلیدواژه‌های انگلیسی مقاله Antibiotic resistance,Coagulase gene,MRSA,PCR,RFLP
نویسندگان مقاله حسین خوشخرام رودمعجنی | hossein khoshkharam roodmajani
department of bacteriology and virology, faculty of medicine and student research committee.


جمال سروری | jamal sarvari
department of bacteriology and virology, faculty of medicine.


عبدالله بازرگانی | abdollah bazargani
department of bacteriology and virology, faculty of medicine.


محمدرضا kandekar قهرمان | mohammadreza kandekar ghahraman
department of bacteriology and virology, faculty of medicine.


علی نظری عالم | ali nazari alam
department of bacteriology and virology, faculty of medicine.


محمد معتمدی فر | mohammad motamedifar
department of bacteriology and virology, faculty of medicine and shiraz hiv aids research center sharc , shiraz university of medical sciences, shiraz, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/397
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