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Iranian Journal of Microbiology، جلد ۶، شماره ۲، صفحات ۶۸-۷۳
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عنوان انگلیسی Recombinant toxin-coregulated pilus A (TcpA) as a candidate subunit cholera vaccine.
چکیده انگلیسی مقاله Background and Objectives: The toxin co-regulated pilus A (TcpA) has been described as a criti- cal pathogenicity factor of Vibrio cholerae . TcpA is a candidate for making subunit vaccine against chol- era. The aim of this study was to produce a candidate vaccine by expressing recombinant TcpA in E. coli . Materials and Methods: In this study, the toxin co-regulated pilus A gene from EL-Tor, V. cholerae subspecies, was am- plified by PCR and sub-cloned into prokaryotic expression vector pGEX4T1. E. coli BL21 (DE3) was transformed with pGEX4T1- TcpA and gene expression was induced by IPTG and purified by GST resin. The integrity of the product was confirmed by Western blot analysis using a standard rabbit anti- V. cholerae antibody. Sera reactivity of infected individuals was further analyzed against the recombinant TcpA protein. Results: The concentration of purified recombinant protein was calculated to be 8 mg/L of initial culture. The in- tegrity of product was confirmed by Western blot analysis using a standard rabbit anti V. cholerae antibody. Sera re- activity of infected individual was further analyzed against the recombinant TcpA protein. The obtained data in- dicated that recombinant TcpA protein from V. cholerae was recognized by patient serum and animal sera. Conclusion: These results show that the recombinant TcpA is antigenic and could be used in a carrier host as an oral vaccine against cholera.
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نویسندگان مقاله سمیه کیایی | somayeh kiaie
department of microbiology and immunology, school of medicine, arak university of medical sciences, arak, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

حمید ابطحی | hamid abtahi
department of microbiology and immunology, school of medicine, arak university of medical sciences, arak, iran and molecular and medicine research center, arak university of medical sciences, arak, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

قاسم مسیبی | ghasem mosayebi
molecular and medicine research center, arak university of medical sciences, arak, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

محمد یوسف علیخانی | mohammadyosef alikhani
department of microbiology, school of medicine, hamedan university of medical sciences, hamedan, iran.


ایرج پاکزاد | iraj pakzad
clinical microbiology research center, ilam university of medical sciences, ilam, iran and department of microbiology, school of medicine, ilam university of medical sciences, ilam, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/453
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