این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۶، شماره ۱، صفحات ۴۱-۴۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Inactivation of model viruses suspended in fresh frozen plasma using novel methylene blue based device.
چکیده انگلیسی مقاله Background and Objective: There is a concern on safety of human Fresh Frozen Plasma (FFP) as it is a source of some medicinal products. The possibility of transmission of blood-borne are reported often due to emerging viruses. There are some Pathogen Reduction Technologies (PRT) to inactivate viruses. Methylene Blue (MB) based method is one of them. The aim of this study was to examine new designated device to inactivate model viruses. Materials and Methods: Four model viruses were used in this study:Vesicular stomatitis virus (VSV), Herpes Simplex Virus I (HSV-1), Bovine Viral DiarrheaVirus(BVDV) and Polio Virus.50% Tissue Culture Infective Dose (TCID 50) and Reed-Muench Methods were used to titer the viruses. MB in two final concentration of 0.1 μM and 1 μM and illumination in about 627nm with red LED (Lamp Emitting Diode) for 15, 30, 45 and 60 minutes were used. Three replicates employed for each experiments. Results: 1μMconcentration of MB showed more effective than 0.1μMin all designed illumination period for inactivation of HSV, VSV and BVDV. This method also demonstrated best results for enveloped model viruses. The most Log reduction for HSV, VSV and BVDV were6.28, 5.54 and 6.22, respectively. For HSV and BVDV inactivation, the best illumination period was 45 minutes. Conclusion: Model viruses showed sensitivity combination of MB and illumination using red LEDs. As results show this device could inactivate model viruses and reduce their titer very close to approved commercial devices, in compare.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله آمنه الیکایی | ameneh elikaei
department of microbiology, faculty of biological science shahidbeheshti university gc,tehran, iran.


زهره شریفی | zohreh sharifi
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran.

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

سید مسعود حسینی | seyed masoud hosseini
department of microbiology, faculty of biological science shahidbeheshti university gc,tehran, iran.


حمید لطیفی | hamid latifi
laser and plasma research institute, shahidbeheshti university, gc, tehran, iran.


میر کامران موسوی حسینی | mir kamaran musavi hosseini
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran.

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/462
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات