این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۵، شماره ۴، صفحات ۳۸۳-۳۹۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Evaluation of FLASH - PCR forrapid detection of Mycobacterium tuberculosis from clinical specimens.
چکیده انگلیسی مقاله Background and Objectives: Tuberculosis (TB) is the oldest known bacterial disease in humans. Due to the rise of morbidity in recent years, early diagnosis of the disease is necessary. Materials and Methods: In this study we used Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR to target IS6110 for rapid detection of M. tuberculosis (MTB). To investigate the important factors influencing the risk of TB, data from patients and their medical records were analyzed. Result: The sensitivity and specificity of FLASH-PCR for detecting MTB were determined as 93.33% and 92.5%, respectively. The findings of this study have suggested that removal of the contaminants in FLASH-PCR sign ificantly reduced the detection time, and MTB was much more rapidly detected in the clinical specimens compared to the conventional culture and smear examination. Results of the medical survey showed that the majority of TB patients were males, over 51 years old, smokers, with pulmonary TB and normal chest X-ray (CXR). Conclusion: MTB can be rapidly detected inclinical specimens using FLASH-PCR in comparison with culture and smear examination.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله عباس علی ایمانی فولادی | abbas ali imani fooladi
applied microbiology, research center baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

یوسف tarvedi زاده | yousef tarvedi zadeh
department of biology, faculty of basic sciences, imam hussein university, tehran, iran.


رضوان مهراب | rezvan mehrab
applied microbiology, research center baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

راحله حلبیان | raheleh halabian
applied microbiology, research center baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

تقی عزیزی | taghi azizi
department of pathology, schoo of medical sciences, baqiyatallah university of medical sciences,tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/496
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات